CALIN JIANU, CORINA MISCA, GEORGETA POP, LAURA - CRISTINA RUSU, LAVINIA ARDELEAN, ALEXANDRA TEODORA GRUIA<br>Chemical Composition and Antimicrobial Activity of Essential <br>Oils Obtained from Dill (Anethum graveolens L.) <br>Grown in Western Romania

CALIN JIANU, CORINA MISCA, GEORGETA POP, LAURA - CRISTINA RUSU, LAVINIA ARDELEAN, ALEXANDRA TEODORA GRUIA
Chemical Composition and Antimicrobial Activity of Essential
Oils Obtained from Dill (Anethum graveolens L.)
Grown in Western Romania

Abstract:

In this study essential oils from inflorescences, stems, immature and mature seeds of dill (Anethum graveolens L.) grown in western Romania (Bucovăţ, Timiş County) were isolated by steam distillation. The isolated essential oils were analyzed by gas chromatography coupled with mass spectrometry (GC-MS) and their antimicrobial activity was evaluated on an array of seven microorganisms: Shigella flexneri (ATCC 12021), Klebsiella pneumoniae (ATCC 13882), Salmonella typhimurium (ATTC 14028), Staphylococcus aureus (ATCC 25923), E. coli (ATCC 25922), Streptococcus pyogenes (ATTC 19615), Clostridium perfringens (ATCC13124). The main component of the oil from mature seeds is carvone 52.37% and limonene 39.20%, the concentration of the latter being slightly lower than in the oil obtained from immature seeds 40.69%, which also contains 34.62% carvone. The content of phellandrene varies in the oil samples analyzed: 12.61% in inflorescences, 3.67% in immature seeds, 30.45% in stems and 2.29% in mature seeds. Significant antimicrobial activity (tested by the Kirby-Bauer method) was recorded against Shigella flexneri, Klebsiella pneumoniae, Salmonella and E. Coli, while no inhibitory effects were observed against Streptococcus pyogenes and Staphylococcus aureus. Keywords: dill, essential oil, steam distillation, GC-MS analysis, antimicrobial activity

Issue: 2012, Volume 63, Issue 6

Pages:

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