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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2012, Volume 63, Issue 9,





Iuliana Stoicescu, Antonela Popescu, Rodica Sirbu, Cosmin Rosca,Dragos Nicolae Doicescu,Vasile Bendic, Camelia Bala
Spectrophotometric Method for Polyphenols Analysis
Validation and application on Trapa Natans L. Species

Abstract:

The objective of this work is to validate the spectrophotometric method to detect and quantify the total polyphenols from different sources. The validation procedure was applied to evaluate the total polyphenols of Trapa natans L. species by the UV-VIS spectrophotometric method with Folin - Ciocalteu reagent, as a chromogenic agent. The method was validated as per ICH rules for linearity, detection limit, quantification limit, precision, and accuracy. Calibration curves show a linear relationship between the absorbance and the concentration. The linearity concentration range was 1.0 to 6.0mg/mL with the correlation coefficient of 0.9994. The limit of detection and limit of quantitation values were found to be 0.2096 mg/mL and 0.6987 mg/mL. We verified the performance parameters after experimental studies and we established that the spectrophotometric method can be applied for the determination of total polyphenols in water caltrop roots, leaves, hulls and pulp hidroalcoolic extracts. Keywords: spectrophotometric, validation, total polyphenol


Issue: 2012, Volume 63, Issue 9
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