RUSANDICA STOICA, RALUCA MADALINA SENIN, RODICA-MARIANA ION<br>Ethanol Concentration Effect on the Extraction of Phenolic <br>Compounds from Ribes nigrum Assessed by Spectrophotometric <br>and HPLC-DAD Methods

RUSANDICA STOICA, RALUCA MADALINA SENIN, RODICA-MARIANA ION
Ethanol Concentration Effect on the Extraction of Phenolic
Compounds from Ribes nigrum Assessed by Spectrophotometric
and HPLC-DAD Methods

Abstract:

The purpose of this study was to assess the phenolic compounds extraction from black currants (Ribes nigrum) by analyzing the effect of ethanol concentration (40-80 % v/v) on the polyphenols obtained. Total phenolic content (TPC) of the extracts, expressed as gallic acid equivalents (GAE), was investigated by the Folin-Ciocalteau method and the total monomeric anthocyanin content (TA), calculated as cyanidin 3-rutinoside equivalents (Cyd-3-rut), by pH-differential method. In order to determine the radical scavenging activities (RSA) of the extracts, DPPH scavenging assay was performed. The spectrophotometric analysis of the 70 % aqueous ethanol extract presented the highest values for TPC (3136.6 mg GAE/100 g), TA (182.4 mg Cyd-3-rut /100 g) and RSA (94.7 %). The quantification of the quercetin-3-rutinoside and the quercetin was carried out with a rapid method of reverse phase high-performance liquid chromatography (RP-HPLC). The obtained results through HPLC analysis confirmed the noticed tends using spectrophotometric analysis. Keywords: total phenolic content, DPPH, cyanidin 3-rutinoside, HPLC, black currants

Issue: 2013, Volume 64, Issue 6

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