Ruxandra Sava Rosianu, Angela Codruta Podariu, Clara Matei, Mircea Tampa, Roxana Oancea<br>Investigation of a Hydroxyapatite/tri-calcium-phosphate <br>Scaffold as Carrier for Mesenchymal Stem Cells in Alveolar Bone Regeneration

Ruxandra Sava Rosianu, Angela Codruta Podariu, Clara Matei, Mircea Tampa, Roxana Oancea
Investigation of a Hydroxyapatite/tri-calcium-phosphate
Scaffold as Carrier for Mesenchymal Stem Cells in Alveolar Bone Regeneration

Abstract:

The aim of this study was to asses alveolar bone repair by allogeneic mesenchymal stem cells using synthetic hydroxyapatite (HA)/tricalcium phosphate (TCP) granular scaffolds as a carrier for alveolar bone tissue engineering. Rat mesenchymal stem cells were obtained from iliac crest bone marrow of Sprague-Dawley rats and expanded without differentiation. Cells were resuspended at a final concentration of 5×106 cells/mL, half in simple osteogenic differentiation medium and the other half in BMP4 - enriched medium on a synthetic HA/TCP scaffold. After another 7 days they were characterized in vitro to prove the viability of the implanted cells. The structures were further implanted in the surgically created bone defects. After 4 weeks, cell viability and differentiation was evaluated by histological analysis. After 4 weeks, cells presented an obvious change in their aspect, having a round shape, polygonal, having a tendency to conflict in concentric circles. Cell cultures with or without BMP contained 58.25 ±18.43% or 43.35 ± 17.68% bone area in vitro (p = 0.049), respectively. There was no statistically significant difference in percent bone area when cells in simple osteogenic differentiation medium or BMP4 enriched medium placed on HA/TCP scaffolds were compared at either time point. The HA/TCP scaffolds allowed for the delivery of allogeneic mesenchymal stem cells in a clinically manageable form that enhanced bone formation at early stages of alveolar repair. Keywords:synthetic hydroxyapatite (HA), tricalcium phosphate (TCP), stem cells

Issue: 2014, Volume 65, Issue 9

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