TUDOR PETREUS, ANA MARIA HOLICOV, GHEORGHE GH. BALAN, DANA ELENA MITRICA<br>Comparative Evaluation of Biocompatibility Assays used in Polymer Biomaterials Assessment

TUDOR PETREUS, ANA MARIA HOLICOV, GHEORGHE GH. BALAN, DANA ELENA MITRICA
Comparative Evaluation of Biocompatibility Assays used in Polymer Biomaterials Assessment

Abstract:

Most of the biocompatibility assays - metabolic, DNA quantitation and membrane permeability - are commonly used to evaluate biomaterials with medical and dental applications. Comparing the results and efficacy among these various protocols may lead to a faster and more accurate choice for a specific assay according to the evaluated biomaterial and its physical/chemical features. The aim of the present work was to perform a reliability comparison between metabolic, membrane integrity and DNA content assays, with respect to biocompatibility of biomaterials with biomedical use. Using chitosan films as polymer support we have observed a good correlation (0.7[R2[1) between cell proliferation evaluated by MTT assay and the DNA quantitation for both tumor and normal phenotype cell lines. For all investigated cell lines grown on chitosan films, less epithelial phenotype MCF10A, there was a good correlation for the two metabolic assays, MTT and CellTiterBlue. Same good correlation was noticed for SytoxGreen (membrane integrity assay) and CyQuant (DNA quantitation). Correlation study performed for metabolic/membrane integrity/DNA quantitation assays demonstrated the usefulness of a permanent adaptation of the cytotoxicity assay for biomaterial evaluation according to physical/chemical and mechanical sample features. For the polymer films used in the present study (chitosan), metabolic assays and DNA quantitation assays showed well correlated results for both tumor and normal phenotype cells. Both assay types may be used, but the final choice will respect potential interferences of the reagents in the assays with biomaterial compounds. Keywords: biocompatibility, biomaterials, metabolic/membrane integrity/DNA quantitation assays, chitosan film, normal phenotype cells

Issue: 2015, Volume 66, Issue 5

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