CLAUDIA VALENTINA POPA, ILEANA CORNELIA FARCASANU, ALEXANDRU CHIVULESCU, MIHAELA BADEA - DONI, MIHAELA - CARMEN CHEREGI, ANDREI FLORIN DANET<br>Optimization of a FIA-CL Method using Luminol and Co(II) Catalyst <br>in Presence of a Chelator for Total Antioxidant Capacity Determination

CLAUDIA VALENTINA POPA, ILEANA CORNELIA FARCASANU, ALEXANDRU CHIVULESCU, MIHAELA BADEA - DONI, MIHAELA - CARMEN CHEREGI, ANDREI FLORIN DANET
Optimization of a FIA-CL Method using Luminol and Co(II) Catalyst
in Presence of a Chelator for Total Antioxidant Capacity Determination

Abstract:

The influence of some important experimental variables on the analytical signals of a flow injection analysis (FIA) method with chemiluminescence (CL) detection (FIA-CL) based on the use of the system luminol/Co(II)/EDTA/H2O2 was reported. The principle and application of the method for the determination of total antioxidant capacity (TAC) of different samples were described in several papers. However further studies on the method are necessary to avoid important errors in determinations. The following experimental variables of the FIA-CL method were studied and optimized: the flow rates of the reagents, the ratio of Co(II)/EDTA in the reagents, the injected sample volume, the concentrations of ethanol, methanol and acetone in the analyzed samples and the concentration of ethanol in the carrier. A calibration graph in different experimental conditions was traced by using caffeic acid as a standard. The optimized method was applied for total antioxidant capacity determination of several ethanolic extracts from nine Lamiaceae species. Keywords: chemiluminescence analysis; flow injection; Co(II); luminol; total antioxidant capacity; plant extracts; caffeic acid

Issue: 2015, Volume 66, Issue 9

Pages:

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)