The in vitro Effects of Several Products of Dental Use on Tumor Cell Lines

Popovici, Ramona Amina; Rusu, Laura Cristina; Podariu, Angela Codruta; Motoc, Ovidiu; Motoc, Veronica; Dehelean, Cristina; Pinzaru, Iulia

Ramona Amina Popovici, Laura Cristina Rusu, Angela Codruta Podariu, Ovidiu Motoc, Veronica Motoc, Cristina Dehelean, Iulia Pinzaru

The in vitro Effects of Several Products of Dental Use on Tumor Cell Lines

Abstract:

Chlorhexidine continues to be considered the gold standard in antiseptic treatment due to its high bactericidal activity. However, some studies reveal the fact that this compound may have some adverse effects both on tissues and cells at standard concentration frequently used in medical practice. Solutions Chlumsky and Walkhoff have a remarkable activity in the infection treatment, but the phenol and chlorophenol, respectively, are substances with a real toxic potential. The purpose of this study was to evaluate the effects of different products of dental use on viability of two tumor cell lines: A375 - human melanoma cells and B164A5 - murine melanoma cells. The dental solutions used in this study were: Gluco-Chex - Chlorhexidine digluconate, Chlumsky and Walkhoff solutions. In order to evaluate the effects of this solutions on cells viability, we tested 3 different concentrations for each solution according to the literature, as follows: Chlorhexidine digluconate (0.000025, 0.00025 and 0.0025%), Chlumsky (0.001, 0.01 and 0.1%) and Walkhoff (0.001, 0.01 and 0.1%). The cells were stimulated for 4 and 24 h and the viability was determined by the means of Trypan Blue and Alamar Blue assays.

Keywords:
chlorhexidine; Chlumsky solution; Walkhoff solution; A375; B164A5

Issue: 2016, Volume 67, Issue 3

Pages:

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This article is published under the Creative Commons Attribution 4.0 International License

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