DIANA CRISTEA, CLAUDIA VALENTINA POPA, SIMONA CARMEN LITESCU, ILEANA CORNELIA FARCASANU, ANDREI  FLORIN DANET<br>Antioxidant Assay for Fruit Juices by Using a Chemiluminescence Method Based on the  Co(II)EDTA/luminol/H2O2 System

DIANA CRISTEA, CLAUDIA VALENTINA POPA, SIMONA CARMEN LITESCU, ILEANA CORNELIA FARCASANU, ANDREI FLORIN DANET
Antioxidant Assay for Fruit Juices by Using a Chemiluminescence Method Based on the Co(II)EDTA/luminol/H2O2 System

Abstract:

It is studied a batch analytical method based on the system Co(II)EDTA/luminol/H2O2 for the determination of antioxidant capacity of different types of sample. The method is based on the oxidation and chemiluminescence of luminol under the influence of reactive oxygen species produced in a Fenton type reaction of hydrogen peroxide with Co(II). The antioxidants from a sample react with the reactive oxygen species and decrease the chemiluminescence signal. It was drawn a calibration graph by plotting the ratio of chemiluminescence intensity in the absence and in the presence of an antioxidant and the concentration of a standard antioxidant (gallic acid). The calibration graph is linear in the domain 10--6 - 10 -4 M galic acid. The method was applied for the analysis of three types of fruit juices. It has been done a comparison between the results for antioxidant capacity determination obtained with the developed method and the literature data for similar types of samples. The acuracy of the method was verified by using standard addition method. Keywords: chemiluminescence analysis; Co(II); luminol; total antioxidant capacity; fruit juice

Issue: 2016, Volume 67, Issue 5

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