RusAndica Stoica, Raluca Senin, Elena Radu, Mihaela Manolache, Florin Oancea, Marian CeauSu <br>Analysis of the Storage Stability of the Wheatgrass <br>(Triticum Aestivum L.) Juice in Terms of Antioxidant Activity <br>and Their Polyphenols Content

RusAndica Stoica, Raluca Senin, Elena Radu, Mihaela Manolache, Florin Oancea, Marian CeauSu
Analysis of the Storage Stability of the Wheatgrass
(Triticum Aestivum L.) Juice in Terms of Antioxidant Activity
and Their Polyphenols Content

Abstract:

A broad spectrum of of health benefits have been attributed to wheatgrass. The fresh juice has high chlorophyll content, essential vitamins (A, B, C, E and K), minerals (iron, calcium, and magnesium), enzymes, amino acids, phenolic compounds (ferulic acid and vanillic acid), and dietary fibers [1].The objective of this work was to evaluate the stability of the wheatgrass juice through the total polyphenol content (mg, gallic acid equivalents, GAE/g, determined according to the Folin-Ciocalteau method) and the antioxidant activity (mg, Trolox equivalent antioxidant capacity, TEAC/g determined by the 1,1-diphenyl-2-picrylhydrazyl free-radical scavenging assay). The sample was divided into four subsamples of which one (E1) was analyzed immediately and the others were kept at -200C until analysis 11, 21 and 32 days, respectively. The stability assessment was carried out by the difference between the subsample averages, analyzed at the set time, and the E1 subsample average. Through statistical method for analysis of variance ANOVA, the standard deviation between subsamples and the repeatability standard deviation were determined on the basis of which was established the detection limit of the stability test. The limit of detection of instability for total phenolic content (TPC) was 0.08 mg GAE/g and of antioxidant activity (AA) was 0.11 mg TEAC/g. Initial properties of the sample are: TPC = 2.15 mg GAE /g and AA = 1.17 mg TEAC /g. The samples stored at -20 0C for 32 days shows instability towards initial properties. Keywords: wheatgrass juice; total polyphenol content; Trolox equivalent antioxidant capacity, stability

Issue: 2016, Volume 67, Issue 9

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