TUDOR PETREUS, HUSAM ABUELBA, ALINA CHELMUS, GHEORGHE G. BALAN, DANA ELENA MITRICA, ELENA TOADER<br>Curcumin Effect on Invasive Behaviour of <br>Triple-negative Breast Cancer Cells

TUDOR PETREUS, HUSAM ABUELBA, ALINA CHELMUS, GHEORGHE G. BALAN, DANA ELENA MITRICA, ELENA TOADER
Curcumin Effect on Invasive Behaviour of
Triple-negative Breast Cancer Cells

Abstract:

Curcumin inhibits cell proliferation, metastasis and angiogenesis and also initiates apoptosis; beside these well documented effects, curcumin influences adhesive proteins behaviour in tumor cells. The aim of the present study was to evaluate the dose response effect of curcumin in continuous/discontinuous treatment on the invasive ability of mesenchymal phenotype triple negative breast cancer cells (MDA-MB-231) versus breast cancer cells expressing E-cadherin (MCF-7). Wound closure for MDA-MB-231 cells was noticed to be better for discontinuous curcumin treatment while for the continuous treatment (48+96h) the wound closure was impaired on more extended areas; this observation supports the curcumin involvement in reversing EMT, by inhibiting the invasive phenotype of these cancer cells. All observations were consistent either for discontinuous (48h) and continuous treatment (48+96h). Differences between wound healing rates in the two depicted conditions are enhanced for curcumin concentrations ranged from 7-10µM. Cell viability determined by Cell-Titer-Glo assay showed values between 80.1-97.2% for all curcumin dosage used, including the continuous treatment. The present study shows the low-dose curcumin effect on the migratory ability of triple negative breast cancer cells in culture. The results emphasize the differences between continuous and intermittent treatment of breast cancer cell lines with curcumin concentrations ranged from 1-10 mM. The best results, with consistent correspondence between migration ability inhibition without reducing cell viability below 95% were obtained by a continuous treatment with 5 mM curcumin Keywords: diferuloylmethane, a6b4 integrin, nuclear factor kappa B, Cell membrane glycoproteins, angiogenesis, apoptosis

Issue: 2016, Volume 67, Issue 9

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