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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2016, Volume 67, Issue 10,





NICOLETA CORINA PREDESCU, CAMELIA PAPUC, VALENTIN NICORESCU, IULIANA GAJAILA , GHEORGHE VALENTIN GORAN, CARMEN DANIELA PETCU, GEORGETA STEFAN
The Influence of Solid-to-Solvent Ratio and Extraction Method
on Total Phenolic Content, Flavonoid Content and Antioxidant Properties of Some Ethanolic Plant Extracts

Abstract:

The purpose of this study was to determine to what extent plant material, solid-to-solvent ratio, and extraction method influence the content of total phenolics and flavonoids and the antioxidant activity of ethanolic extracts obtained from dried fruits of dog-rose, sea buckthorn and hawthorn. The extractions were performed by maceration, Soxhlet and ultrasound-assisted methods, with 60% ethanol, and the solid-to-solvent ratios used were 1/5 and 1/10 (w:v). For each extract it was determined the total phenolic content (TPC), the flavonoid content (FC), the ability to scavenge DPPH•, Fe3+ reducing power, and also the ability of chelating Fe2+. The highest total phenolics and flavonoids contents were found in dog-rose fruits extracts. Maceration and ultrasound-assisted extraction methods led to the highest concentrations of phenolics and flavonoids, and solid-to-solvent ratio 1/10 (w:v) was the most effective. The extracts of dog-rose fruits showed the highest DPPH• scavenging activity and Fe3+ reducing power, and the hawthorn fruits extracts registered the highest capacity of chelating Fe2+. Key words: phenolics, flavonoids, solid-to-solvent ratio, extraction method


Issue: 2016, Volume 67, Issue 10
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