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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2016, Volume 67, Issue 10,





ADRIANA MONICA CHIS, CORNELIA PURCAREA
Versatility of Folin-Ciocalteu Method Applied on Honey
Determination of Total Phenols

Abstract:

The main benefits of honey regarding human health issue from its minor components mainly because of their antioxidant properties. So far the studies emphasise the phenolic compounds beneficial effect related to the antioxidant activity of honey. The aim of this study was to verify the influence of temperature and reaction time on honey total phenol content (TPC) value determined using Folin-Ciocalteu assay. Twenty samples of floral and honeydew honey were tested for total phenol content in four experimental variants: A -at 20oC for 2 h, B - 20oC for 1 h, C - 40oC for 20 min and D - 4oC for 30 min . The comparison of A variant, the most spread one, to the others shows that the reduction of time from 2 h to 1 h leads to non-significant differences while the rise of temperature, even for short laps of time, reveals significant differences between the recorded TPC values. Keywords: honey, phenols, Folin-Ciocalteu, time, temperature


Issue: 2016, Volume 67, Issue 10
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