IRINA LUPESCU, MIHAELA CARMEN EREMIA, GABRIELA VALERIA SAVOIU, MARIA SPIRIDON, DENIS PANAITESCU, CRISTIAN NICOLAE, MARIANA GRATIELA VLADU, AMALIA STEFANIU<br>Comparative Studies on Isolation of Medium-chain-length Polyhydroxyalkanoates Produced by Pseudomonas spp. Strains<br><br>

IRINA LUPESCU, MIHAELA CARMEN EREMIA, GABRIELA VALERIA SAVOIU, MARIA SPIRIDON, DENIS PANAITESCU, CRISTIAN NICOLAE, MARIANA GRATIELA VLADU, AMALIA STEFANIU
Comparative Studies on Isolation of Medium-chain-length Polyhydroxyalkanoates Produced by Pseudomonas spp. Strains

Abstract:

Several methods were used for recovering medium-chain-length polyhydroxyalkanoates (mcl-PHAs) biosynthesized by a Pseudomonas spp. strain cells, in a fermentation medium based on sodium citrate and sodium octanoate. To improve bacterial cells separation, flocculation was tested, using a anionic polyelectrolyte. Post-biosynthesis processing of separated biomass included cell rupture and solubilization of all cell materials except PHAs and/or solvent extraction of PHAs. NaOCl or lysozyme + EDTA were used for biomass digestion, CHCl3 and acetone for polyester extraction. The isolated polymers were characterized by GC-FID, FTIR, and DSC-TGA. The best results were obtained using acetone Soxhlet extraction from dried cells. Thus, mcl-PHAs were recovered as thin polymeric membranes, of above 99% purity, representing 44-54% of dry bacterial cells, and containing C6, C8 and C10 hydroxyacid monomers in a gravimetric percentage of: 9.50 -11.47% C6, 85.25 - 86.75% C8 and 1.85 - 3.01% C10. Keywords: Medium chain length polyhydroxyalkanoates; Pseudomonas spp., extraction

Issue: 2016, Volume 67, Issue 10

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