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https://doi.org/10.37358/Rev.Chim.1949

OSIM Nr. R102355
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Revista de Chimie (Rev. Chim.), Year 2016, Volume 67, Issue 11,





CRISTINA OANA RADUCANU, LILIANA CHELARU, LAURENTIU SIMION, MARCEL COSTULEANU
Biochemical Effects of Biological Supports-Included
Stem Cells on Eye Cells Development

Abstract:

Mesenchymal stem cells are considered a promising tool to help the repairing of ocular surface injuries, although it is not well established their possibility to be derived toward corneal epithelial cells. The pathways through which they communicate with or influence the turnover of other cell types are not well known. The aim of our studies was represented by the influences exerted by growth conditions on rat mesenchymal stem cell abilities to modulate the development of rat corneal epithelium cells. From this point of view, we compared the mesenchymal stem cells grown on a 3D polystyrene scaffold and those grown on classic culture dishes. The clear results are that the mesenchymal stem cells grown on a three-dimensional structure are more potent in influencing the development of rat corneal epithelium cells in vitro. Thus, the establishment of these last cells as a monolayer and their capacity to proliferate for many passages as well as the fine mechanisms of achieving these abilities are to be established by further studies. Keywords: mesenchymal stem cell, corneal epithelial cell, rat, three-dimensional culture, in vitro


Issue: 2016, Volume 67, Issue 11
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