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Revista de Chimie (Rev. Chim.), Year 2017, Volume 68, Issue 1, 163-167

https://doi.org/10.37358/RC.17.1.5411

Luminita Lazar, Cristina Ioana Bica, Krisztina Martha, Mariana Pacurar, Eugen Bud, Ana Petra Lazar, Lilla Lorinczi

The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease

Abstract:

Polymerase chain reaction (PCR) shows a high specificity and allows us to identify pathogenic periodontal bacteria. We chose 45 patients wich were divided into three groups with various types of treatment: (I) - SRP; (II) - SRP, followed by topical application of antiseptics (SRP + local); (III) - SRP followed by systemic administration of antimicrobial agents (SRP + systemic). We collected samples from the initial time (TO) and one month after the treatment (T1) for each patient. For the microbiological assessment of periodontal therapy, we analyzed 90 subgingival plaque samples using PCR technique which provides qualitative data on five periodontopathogenic bacteria species: A. actinomycetemcomitans, P.gingivalis, P.intermedia, T.forsythia, and T.denticola. The treatment was followed by a qualitative change of the bacteria detected previously in a different ratio depending on the treatment. We found the inefficiency of mechanical treatment regarding the reduction of periodontal bacteria in patients belonging to group I, an improvement in the results of group II, while the treatment in group III proved to be the most effective . In patients detected A.a+ and/ or P.g+ a systemic antibiotic treatment is required because these periodontal bacteria penetrate the tissue and mucosal surfaces of the oral cavity.
Keywords:
PCR; periodontopathogenic bacteria; periodontal treatment

Issue: 2017, Volume 68, Issue 1
Pages: 163-167
Publication date: 15/02/2017
https://doi.org/10.37358/RC.17.1.5411
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
LAZAR, L., BICA, C.I., MARTHA, K., PACURAR, M., BUD, E., LAZAR, A.P., LORINCZI, L., The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease, Rev. Chim., 68(1), 2017, 163-167.

Vancouver
Lazar L, Bica CI, Martha K, Pacurar M, Bud E, Lazar AP, Lorinczi L. The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease. Rev. Chim.[internet]. 2017 Jan;68(1):163-167. Available from: https://doi.org/10.37358/RC.17.1.5411


APA 6th edition
Lazar, L., Bica, C.I., Martha, K., Pacurar, M., Bud, E., Lazar, A.P. & Lorinczi, L. (2017). The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease. Revista de Chimie, 68(1), 163-167. https://doi.org/10.37358/RC.17.1.5411


Harvard
Lazar, L., Bica, C.I., Martha, K., Pacurar, M., Bud, E., Lazar, A.P., Lorinczi, L. (2017). 'The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease', Revista de Chimie, 68(1), pp. 163-167. https://doi.org/10.37358/RC.17.1.5411


IEEE
L. Lazar, C.I. Bica, K. Martha, M. Pacurar, E. Bud, A.P. Lazar, L. Lorinczi, "The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease". Revista de Chimie, vol. 68, no. 1, pp. 163-167, 2017. [online]. https://doi.org/10.37358/RC.17.1.5411


Text
Luminita Lazar, Cristina Ioana Bica, Krisztina Martha, Mariana Pacurar, Eugen Bud, Ana Petra Lazar, Lilla Lorinczi,
The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease,
Revista de Chimie,
Volume 68, Issue 1,
2017,
Pages 163-167,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.1.5411.
(https://revistadechimie.ro/Articles.asp?ID=5411)
Keywords: PCR; periodontopathogenic bacteria; periodontal treatment


RIS
TY - JOUR
T1 - The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease
A1 - Lazar, Luminita
A2 - Bica, Cristina Ioana
A3 - Martha, Krisztina
A4 - Pacurar, Mariana
A5 - Bud, Eugen
A6 - Lazar, Ana Petra
A7 - Lorinczi, Lilla
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 1
SP - 163
EP - 167
UR - https://doi.org/10.37358/RC.17.1.5411
KW - PCR
KW - periodontopathogenic bacteria
KW - periodontal treatment
ER -


BibTex
@article{RevCh2017P163,
author = {Lazar Luminita and Bica Cristina Ioana and Martha Krisztina and Pacurar Mariana and Bud Eugen and Lazar Ana Petra and Lorinczi Lilla},
title = {The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease},
journal = {Revista de Chimie},
volume = {68},
number = {1},
pages = {163-167},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.1.5411},
url = {https://revistadechimie.ro/Articles.asp?ID=5411}
}
 
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