Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features

Mitache, Mihaela Magdalena; Curutiu, Carmen; Rusu, Elena; Bahna, Ramona; Ditu, Mara; Moldovan, Horatiu; Hancu, Violeta; Chifiriuc, Mariana Carmen

Mihaela Magdalena Mitache, Carmen Curutiu, Elena Rusu, Ramona Bahna, Mara Ditu, Horatiu Moldovan, Violeta Hancu, Mariana Carmen Chifiriuc

Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features

Abstract:

One of the most frequent chronic complications occurred in diabetes patients are the urinary tract infections (UTIs). This study aimed to investigate the incidence of UTIs in a cohort of 93 (47 males: 46 females) diabetic patients, the prevalence of different microbial species involved and their virulence and antibiotic resistance profiles. The identification of the uropathogenic strains in the positive urine samples was performed using conventional methods and API tests. After identification, the antibiotic susceptibility profiles were established by the standardized disk diffusion method and double disk diffusion test was performed for the confirmation of ESBL and inducible AmpC b -lactamase phenotypes. The isolated strains were tested for the production of different cell associated and soluble virulence factors, i.e.: bacterial adherence to cellular substrata (HeLa cells), hemolysins (hemolysis spot, CAMP-like), amylase, caseinase, aesculin hydrolysis, DNA-ase, lipase and lecithinase. In the analyzed group, the total prevalence of UTIs was of 46%, a higher incidence being observed in the female patients (64%). Similar to other studies, the etiology of UTI in the investigated diabetes patients was dominated by E. coli, followed by Klebsiella sp. strains. The isolated strains preserved good susceptibility rates to quinolones and aminoglycosides and revealed important virulence features, related to their capacity to colonize the cellular substratum and to produce soluble virulence factors involved in persistence, colonization and progression of the infectious process. The high percentage of beta-lactam resistant strains (including carbapenem-resistant ones) requires careful surveillance of the dynamics of susceptibility profiles for limiting the emergence of these strains in community.

Keywords:
diabetes; urinary tract infections; resistance; virulence

Issue: 2017, Volume 68, Issue 3

Pages: 566-569

Publication date: 2017/4/15

https://doi.org/10.37358/RC.17.3.5502

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MITACHE, M.M., CURUTIU, C., RUSU, E., BAHNA, R., DITU, M., MOLDOVAN, H., HANCU, V., CHIFIRIUC, M.C., Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features, Rev. Chim., 68(3), 2017, 566-569.

Vancouver
Mitache MM, Curutiu C, Rusu E, Bahna R, Ditu M, Moldovan H, Hancu V, Chifiriuc MC. Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features. Rev. Chim.[internet]. 2017 Mar;68(3):566-569. Available from: https://doi.org/10.37358/RC.17.3.5502

APA 6th edition
Mitache, M.M., Curutiu, C., Rusu, E., Bahna, R., Ditu, M., Moldovan, H., Hancu, V. & Chifiriuc, M.C. (2017). Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features. Revista de Chimie, 68(3), 566-569. https://doi.org/10.37358/RC.17.3.5502

Harvard
Mitache, M.M., Curutiu, C., Rusu, E., Bahna, R., Ditu, M., Moldovan, H., Hancu, V., Chifiriuc, M.C. (2017). 'Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features', Revista de Chimie, 68(3), pp. 566-569. https://doi.org/10.37358/RC.17.3.5502

IEEE
M.M. Mitache, C. Curutiu, E. Rusu, R. Bahna, M. Ditu, H. Moldovan, V. Hancu, M.C. Chifiriuc, "Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features". Revista de Chimie, vol. 68, no. 3, pp. 566-569, 2017. [online]. https://doi.org/10.37358/RC.17.3.5502

Text
Mihaela Magdalena Mitache, Carmen Curutiu, Elena Rusu, Ramona Bahna, Mara Ditu, Horatiu Moldovan, Violeta Hancu, Mariana Carmen Chifiriuc,
Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features,
Revista de Chimie,
Volume 68, Issue 3,
2017,
Pages 566-569,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.3.5502.
(https://revistadechimie.ro/Articles.asp?ID=5502)
Keywords: diabetes; urinary tract infections; resistance; virulence

RIS
TY - JOUR
T1 - Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features
A1 - Mitache, Mihaela Magdalena
A2 - Curutiu, Carmen
A3 - Rusu, Elena
A4 - Bahna, Ramona
A5 - Ditu, Mara
A6 - Moldovan, Horatiu
A7 - Hancu, Violeta
A8 - Chifiriuc, Mariana Carmen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 3
SP - 566
EP - 569
UR - https://doi.org/10.37358/RC.17.3.5502
KW - diabetes
KW - urinary tract infections
KW - resistance
KW - virulence
ER -

BibTex
@article{RevCh2017P566,
author = {Mitache Mihaela Magdalena and Curutiu Carmen and Rusu Elena and Bahna Ramona and Ditu Mara and Moldovan Horatiu and Hancu Violeta and Chifiriuc Mariana Carmen},
title = {Urinary Tract Infections in Patients with Type 1 and Type 2 Diabetes: Etiology, Resistance to Antibacterial Chemicals and Virulence Features},
journal = {Revista de Chimie},
volume = {68},
number = {3},
pages = {566-569},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.3.5502},
url = {https://revistadechimie.ro/Articles.asp?ID=5502}
}

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