Daniela Ionescu, Ancuta Ioana Isaia (Oarcea), Mihai Gafencu, Sorin Bolintineanu, Adina Cata, Daniela Bratosin, Lavinia Toader, Ioana Maria Carmen Ienascu
Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry
Abstract:
This study is aimed to evaluate the inhibitory effect of natural extracts on reactive oxygen species (ROS) in human red cells (RBCs) under oxidative stress and to demonstrate the importance of using natural extracts from plants in the fight against oxidative stress. Therefore, seven nonalcoholic extracts were tested at several dilutions, in order to prove their antioxidant activity using flow cytometry. The level of intracellular hydrogen peroxide significantly decreased when the cells were coincubated with different extracts, the values of the Mean Fluorescence Intensity (MFI) ranging from 206 to 3702, compared with 3872, the value obtained in case of H2O2-treated cells (RBCs + H2O2). All the tested extracts demonstrated a good inhibition, Melissa officinalis L. extract showed a greater percentage of hydrogen peroxide inhibition even at the last dilution (94.70% (D-a)….85.76% (D-e)). The results suggest the importance of the investigated natural extracts to combat oxidative stress.
Cite this article as: IONESCU, D., ISAIA (OARCEA), A.I., GAFENCU, M., BOLINTINEANU, S., CATA, A., BRATOSIN, D., TOADER, L., IENASCU, I.M.C., Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry, Rev. Chim., 68(4), 2017, 652-655.
Vancouver Ionescu D, Isaia (Oarcea) AI, Gafencu M, Bolintineanu S, Cata A, Bratosin D, Toader L, Ienascu IMC. Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry. Rev. Chim.[internet]. 2017 Apr;68(4):652-655. Available from: https://doi.org/10.37358/RC.17.4.5524
APA 6th edition Ionescu, D., Isaia (Oarcea), A.I., Gafencu, M., Bolintineanu, S., Cata, A., Bratosin, D., Toader, L. & Ienascu, I.M.C. (2017). Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry. Revista de Chimie, 68(4), 652-655. https://doi.org/10.37358/RC.17.4.5524
Harvard Ionescu, D., Isaia (Oarcea), A.I., Gafencu, M., Bolintineanu, S., Cata, A., Bratosin, D., Toader, L., Ienascu, I.M.C. (2017). 'Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry', Revista de Chimie, 68(4), pp. 652-655. https://doi.org/10.37358/RC.17.4.5524
IEEE D. Ionescu, A.I. Isaia(Oarcea), M. Gafencu, S. Bolintineanu, A. Cata, D. Bratosin, L. Toader, I.M.C. Ienascu, "Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry". Revista de Chimie, vol. 68, no. 4, pp. 652-655, 2017. [online]. https://doi.org/10.37358/RC.17.4.5524
Text Daniela Ionescu, Ancuta Ioana Isaia(Oarcea), Mihai Gafencu, Sorin Bolintineanu, Adina Cata, Daniela Bratosin, Lavinia Toader, Ioana Maria Carmen Ienascu, Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry, Revista de Chimie, Volume 68, Issue 4, 2017, Pages 652-655, ISSN 2668-8212, https://doi.org/10.37358/RC.17.4.5524. (https://revistadechimie.ro/Articles.asp?ID=5524) Keywords: plant extracts; antioxidant capacity; MFI; ROS inhibition
RIS TY - JOUR T1 - Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry A1 - Ionescu, Daniela A2 - Isaia (Oarcea), Ancuta Ioana A3 - Gafencu, Mihai A4 - Bolintineanu, Sorin A5 - Cata, Adina A6 - Bratosin, Daniela A7 - Toader, Lavinia A8 - Ienascu, Ioana Maria Carmen JF - Revista de Chimie JO - Rev. Chim. PB - Revista de Chimie SRL SN - 2668-8212 Y1 - 2017 VL - 68 IS - 4 SP - 652 EP - 655 UR - https://doi.org/10.37358/RC.17.4.5524 KW - plant extracts KW - antioxidant capacity KW - MFI KW - ROS inhibition ER -
BibTex @article{RevCh2017P652, author = {Ionescu Daniela and Isaia (Oarcea) Ancuta Ioana and Gafencu Mihai and Bolintineanu Sorin and Cata Adina and Bratosin Daniela and Toader Lavinia and Ienascu Ioana Maria Carmen}, title = {Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry}, journal = {Revista de Chimie}, volume = {68}, number = {4}, pages = {652-655}, year = {2017}, issn = {2668-8212}, doi = {https://doi.org/10.37358/RC.17.4.5524}, url = {https://revistadechimie.ro/Articles.asp?ID=5524} }
