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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2017, Volume 68, Issue 4, 768-771

https://doi.org/10.37358/RC.17.4.5549

Alexandra Petrus, Corina Ratiu, Lavinia Noveanu, Rodica Lighezan, Mariana Rosca, Danina Muntean, Oana Duicu

Assessment of Mitochondrial Respiration in Human Platelets

Abstract:

It has been long recognized that the impairment of platelet mitochondrial function occurs in a broad spectrum of diseases. Accordingly, the assessment of platelet respiratory dys/function has emerged as a putative approach allowing the characterization of the early impairment of human bioenergetic profile in several chronic pathologies. The aim of this study was to standardize the methodology for platelet isolation from peripheral blood and the measurement of mitochondrial oxygen consumption by means of high-resolution respirometry, respectively. The platelet isolation protocol consisted of two consecutive centrifugations of the whole blood collected from adult healthy females (n = 10) yielding a platelet-rich plasma sample. Respiration was measured at 370C using the Oxygraph-2k (Oroboros Instruments, Austria) according to a classic substrate-uncoupler-inhibitor-titration protocol. Platelets permeabilized with digitonin were allowed to respire in the presence of complex I (glutamate and malate) and complex II (succinate) substrates. We obtained a respiratory control ratio of 2.77 ± 3.65 that indicates an accurate coupling efficiency of oxidative phosphorylation. The in vitro measurement of platelet respiration is a reliable method to evaluate the bioenergetic profile in humans. The standardized technique will be further used to assess the occurrence of mitochondrial dysfunction in peripheral blood in the setting of various chronic non-communicable diseases.
Keywords:
platelets; mitochondria; high-resolution respirometry

Issue: 2017, Volume 68, Issue 4
Pages: 768-771
Publication date: 2017/5/15
https://doi.org/10.37358/RC.17.4.5549
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
PETRUS, A., RATIU, C., NOVEANU, L., LIGHEZAN, R., ROSCA, M., MUNTEAN, D., DUICU, O., Assessment of Mitochondrial Respiration in Human Platelets, Rev. Chim., 68(4), 2017, 768-771.

Vancouver
Petrus A, Ratiu C, Noveanu L, Lighezan R, Rosca M, Muntean D, Duicu O. Assessment of Mitochondrial Respiration in Human Platelets. Rev. Chim.[internet]. 2017 Apr;68(4):768-771. Available from: https://doi.org/10.37358/RC.17.4.5549


APA 6th edition
Petrus, A., Ratiu, C., Noveanu, L., Lighezan, R., Rosca, M., Muntean, D. & Duicu, O. (2017). Assessment of Mitochondrial Respiration in Human Platelets. Revista de Chimie, 68(4), 768-771. https://doi.org/10.37358/RC.17.4.5549


Harvard
Petrus, A., Ratiu, C., Noveanu, L., Lighezan, R., Rosca, M., Muntean, D., Duicu, O. (2017). 'Assessment of Mitochondrial Respiration in Human Platelets', Revista de Chimie, 68(4), pp. 768-771. https://doi.org/10.37358/RC.17.4.5549


IEEE
A. Petrus, C. Ratiu, L. Noveanu, R. Lighezan, M. Rosca, D. Muntean, O. Duicu, "Assessment of Mitochondrial Respiration in Human Platelets". Revista de Chimie, vol. 68, no. 4, pp. 768-771, 2017. [online]. https://doi.org/10.37358/RC.17.4.5549


Text
Alexandra Petrus, Corina Ratiu, Lavinia Noveanu, Rodica Lighezan, Mariana Rosca, Danina Muntean, Oana Duicu,
Assessment of Mitochondrial Respiration in Human Platelets,
Revista de Chimie,
Volume 68, Issue 4,
2017,
Pages 768-771,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.4.5549.
(https://revistadechimie.ro/Articles.asp?ID=5549)
Keywords: platelets; mitochondria; high-resolution respirometry


RIS
TY - JOUR
T1 - Assessment of Mitochondrial Respiration in Human Platelets
A1 - Petrus, Alexandra
A2 - Ratiu, Corina
A3 - Noveanu, Lavinia
A4 - Lighezan, Rodica
A5 - Rosca, Mariana
A6 - Muntean, Danina
A7 - Duicu, Oana
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 4
SP - 768
EP - 771
UR - https://doi.org/10.37358/RC.17.4.5549
KW - platelets
KW - mitochondria
KW - high-resolution respirometry
ER -


BibTex
@article{RevCh2017P768,
author = {Petrus Alexandra and Ratiu Corina and Noveanu Lavinia and Lighezan Rodica and Rosca Mariana and Muntean Danina and Duicu Oana},
title = {Assessment of Mitochondrial Respiration in Human Platelets},
journal = {Revista de Chimie},
volume = {68},
number = {4},
pages = {768-771},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.4.5549},
url = {https://revistadechimie.ro/Articles.asp?ID=5549}
}
 
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