Assessment of Mitochondrial Respiration in Human Platelets
Abstract:
It has been long recognized that the impairment of platelet mitochondrial function occurs in a broad spectrum of diseases. Accordingly, the assessment of platelet respiratory dys/function has emerged as a putative approach allowing the characterization of the early impairment of human bioenergetic profile in several chronic pathologies. The aim of this study was to standardize the methodology for platelet isolation from peripheral blood and the measurement of mitochondrial oxygen consumption by means of high-resolution respirometry, respectively. The platelet isolation protocol consisted of two consecutive centrifugations of the whole blood collected from adult healthy females (n = 10) yielding a platelet-rich plasma sample. Respiration was measured at 370C using the Oxygraph-2k (Oroboros Instruments, Austria) according to a classic substrate-uncoupler-inhibitor-titration protocol. Platelets permeabilized with digitonin were allowed to respire in the presence of complex I (glutamate and malate) and complex II (succinate) substrates. We obtained a respiratory control ratio of 2.77 ± 3.65 that indicates an accurate coupling efficiency of oxidative phosphorylation. The in vitro measurement of platelet respiration is a reliable method to evaluate the bioenergetic profile in humans. The standardized technique will be further used to assess the occurrence of mitochondrial dysfunction in peripheral blood in the setting of various chronic non-communicable diseases.
Cite this article as: PETRUS, A., RATIU, C., NOVEANU, L., LIGHEZAN, R., ROSCA, M., MUNTEAN, D., DUICU, O., Assessment of Mitochondrial Respiration in Human Platelets, Rev. Chim., 68(4), 2017, 768-771.
Vancouver Petrus A, Ratiu C, Noveanu L, Lighezan R, Rosca M, Muntean D, Duicu O. Assessment of Mitochondrial Respiration in Human Platelets. Rev. Chim.[internet]. 2017 Apr;68(4):768-771. Available from: https://doi.org/10.37358/RC.17.4.5549
APA 6th edition Petrus, A., Ratiu, C., Noveanu, L., Lighezan, R., Rosca, M., Muntean, D. & Duicu, O. (2017). Assessment of Mitochondrial Respiration in Human Platelets. Revista de Chimie, 68(4), 768-771. https://doi.org/10.37358/RC.17.4.5549
Harvard Petrus, A., Ratiu, C., Noveanu, L., Lighezan, R., Rosca, M., Muntean, D., Duicu, O. (2017). 'Assessment of Mitochondrial Respiration in Human Platelets', Revista de Chimie, 68(4), pp. 768-771. https://doi.org/10.37358/RC.17.4.5549
IEEE A. Petrus, C. Ratiu, L. Noveanu, R. Lighezan, M. Rosca, D. Muntean, O. Duicu, "Assessment of Mitochondrial Respiration in Human Platelets". Revista de Chimie, vol. 68, no. 4, pp. 768-771, 2017. [online]. https://doi.org/10.37358/RC.17.4.5549
Text Alexandra Petrus, Corina Ratiu, Lavinia Noveanu, Rodica Lighezan, Mariana Rosca, Danina Muntean, Oana Duicu, Assessment of Mitochondrial Respiration in Human Platelets, Revista de Chimie, Volume 68, Issue 4, 2017, Pages 768-771, ISSN 2668-8212, https://doi.org/10.37358/RC.17.4.5549. (https://revistadechimie.ro/Articles.asp?ID=5549) Keywords: platelets; mitochondria; high-resolution respirometry
RIS TY - JOUR T1 - Assessment of Mitochondrial Respiration in Human Platelets A1 - Petrus, Alexandra A2 - Ratiu, Corina A3 - Noveanu, Lavinia A4 - Lighezan, Rodica A5 - Rosca, Mariana A6 - Muntean, Danina A7 - Duicu, Oana JF - Revista de Chimie JO - Rev. Chim. PB - Revista de Chimie SRL SN - 2668-8212 Y1 - 2017 VL - 68 IS - 4 SP - 768 EP - 771 UR - https://doi.org/10.37358/RC.17.4.5549 KW - platelets KW - mitochondria KW - high-resolution respirometry ER -
BibTex @article{RevCh2017P768, author = {Petrus Alexandra and Ratiu Corina and Noveanu Lavinia and Lighezan Rodica and Rosca Mariana and Muntean Danina and Duicu Oana}, title = {Assessment of Mitochondrial Respiration in Human Platelets}, journal = {Revista de Chimie}, volume = {68}, number = {4}, pages = {768-771}, year = {2017}, issn = {2668-8212}, doi = {https://doi.org/10.37358/RC.17.4.5549}, url = {https://revistadechimie.ro/Articles.asp?ID=5549} }
