Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor

Crisan, Mara; Maria, Gheorghe

Mara Crisan, Gheorghe Maria

Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor

Abstract:

Novel coupled enzymatic systems reported important applications in the industrial bio-catalysis. Multi-enzymatic reactions can successfully replace complex chemical syntheses, using milder reaction conditions, and generating less waste. For such systems acting simultaneously, the model-based engineering calculations (design, reactor operation optimization) are difficult tasks, because they must account for interacting reactions, differences in enzymes optimal activity domains and deactivation kinetics. The determination of the optimal operating mode (enzyme ratios, enzyme feeding policy, temperature, pH) often turns into a difficult multi-objective optimization problem with multiple constraints to be solved for every particular system. The paper focuses on applying a modular screening procedure that can identify the optimal operating policy of an enzymatic reactor, which minimizes the enzyme consumption, given the process kinetic model, and an imposed production capacity. Following an optimization procedure, the process effectiveness is evaluated in a systematic approach, by including simple batch reactor (BR), batch with intermittent addition of the key-enzyme following certain optimal policies (BRP). Exemplification is made for the case of the enzymatic reduction of D-fructose to mannitol by using suspended MDH (mannitol dehydrogenase) and NADH (Nicotinamide adenine dinucleotide) cofactor, with the in-situ continuous regeneration of the cofactor by the expense of formate degradation in the presence of suspended FDH (Formate dehydrogenase).

Keywords:
D-fructose reduction; Enzymatic reactor optimization; D-Mannitol; Mannitol dehydrogenase; Formate dehydrogenase; Coenzyme regeneration

Issue: 2017, Volume 68, Issue 9

Pages: 2196-2203

Publication date: 2017/10/15

https://doi.org/10.37358/RC.17.9.5854

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
CRISAN, M., MARIA, G., Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor, Rev. Chim., 68(9), 2017, 2196-2203.

Vancouver
Crisan M, Maria G. Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor. Rev. Chim.[internet]. 2017 Sep;68(9):2196-2203. Available from: https://doi.org/10.37358/RC.17.9.5854

APA 6th edition
Crisan, M., Maria, G. (2017). Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor. Revista de Chimie, 68(9), 2196-2203. https://doi.org/10.37358/RC.17.9.5854

Harvard
Crisan, M., Maria, G. (2017). 'Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor', Revista de Chimie, 68(9), pp. 2196-2203. https://doi.org/10.37358/RC.17.9.5854

IEEE
M. Crisan, G. Maria, "Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor". Revista de Chimie, vol. 68, no. 9, pp. 2196-2203, 2017. [online]. https://doi.org/10.37358/RC.17.9.5854

Text
Mara Crisan, Gheorghe Maria,
Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor,
Revista de Chimie,
Volume 68, Issue 9,
2017,
Pages 2196-2203,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.9.5854.
(https://revistadechimie.ro/Articles.asp?ID=5854)
Keywords: D-fructose reduction; Enzymatic reactor optimization; D-Mannitol; Mannitol dehydrogenase; Formate dehydrogenase; Coenzyme regeneration

RIS
TY - JOUR
T1 - Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor
A1 - Crisan, Mara
A2 - Maria, Gheorghe
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 9
SP - 2196
EP - 2203
UR - https://doi.org/10.37358/RC.17.9.5854
KW - D-fructose reduction; Enzymatic reactor optimization; D-Mannitol; Mannitol dehydrogenase; Formate dehydrogenase; Coenzyme regeneration
ER -

BibTex
@article{RevCh2017P2196,
author = {Crisan Mara and Maria Gheorghe},
title = {Modular Simulation to Determine the Optimal Operating Policy of a Batch Reactor for the Enzymatic Fructose Reduction to Mannitol with the in situ Continuous Enzymatic Regeneration of the NAD Cofactor},
journal = {Revista de Chimie},
volume = {68},
number = {9},
pages = {2196-2203},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.9.5854},
url = {https://revistadechimie.ro/Articles.asp?ID=5854}
}

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