Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania

Mitache, Mihaela Magdalena; Banu, Otilia; Borcan, Elvira; Gheorghe, Irina; Marutescu, Luminita; Popa, marcela; Predescu, Miruna; Rusu, Elena; Nemes, Roxana; Chifiriuc, Mariana Carmen

Mihaela Magdalena Mitache, Otilia Banu, Elvira Borcan, Irina Gheorghe, Luminita Marutescu,marcela Popa, Miruna Predescu, Elena Rusu, Roxana Nemes, Mariana Carmen Chifiriuc

Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania

Abstract:

We aimed to investigate the antibiotic resistance and virulence markers in Gram negative bacilli (GNB) and Gram positives coccus (GPC), strains recently isolated from the hospital environment and from patients with surgical wound infections in order to obtain epidemiologically relevant data.The strains identification was performed with the automated miniApi system. The resistance phenotypes were established using disk diffusion (CLSI, 2017). 61 strains were screened for the production of enzymatic soluble virulence factors: hemolysins, amylase, caseinase, aesculin hydrolysis, DNA-ase, lipase, gelatinase and lecithinase, which give microorganisms the ability to colonize and disseminate in the host. Multiplex PCR reactions were performed for the detection of carbapenemases, aminoglycoside-resistant determinants (AME’s), quinolone and tetracycline resistance in GNR and SCCmec cassette type in Staphylococcus aureus strains and to identify the genetic support of cell-associated and soluble virulence factors in E. coli strains (fimH, sfaDE, papC, eaea, cnf1, bfpa, eaf, AggR, EaggE genes) and biofilm production in Acinetobacter baumannii isolates (OmpA).The isolated E. coli and A. baumannii strains were resistant to â-lactam antibiotics, including penicillins and beta-lactamase inhibitors, third / fourth generation cephalosporins and carbapenems (encoded by blaOXA-48like and blaTEMlike genes), quinolones (qnrA and qnrB), aminoglycosides (aadB), and tetracyclines (encoded by tetA and tetB). Most of the strains presented at least two of the eight tested virulence factors. The carbapenemases and ESBLs producers proved to be positive for the majority of the tested soluble virulence factors, proving the pathogenic potential of these strains. In S. aureus isolates the molecular analysis showed that 60% of the isolates were MRSA and the molecular analysis revealed the presence of the SCCmec cassette type mec IVa and III types. Our data suggest the hypothesis according to which nosocomial origin of the strains can be explained by multiple drug resistance and virulence determinants.

Keywords:
resistance; virulence; nosocomial infections

Issue: 2018, Volume 69, Issue 11

Pages: 3126-3132

Publication date: 2018/12/15

https://doi.org/10.37358/RC.18.11.6697

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MITACHE, M.M., BANU, O., BORCAN, E., GHEORGHE, I., MARUTESCU, L., POPA, M., PREDESCU, M., RUSU, E., NEMES, R., CHIFIRIUC, M.C., Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania, Rev. Chim., 69(11), 2018, 3126-3132.

Vancouver
Mitache MM, Banu O, Borcan E, Gheorghe I, Marutescu L, Popa m, Predescu M, Rusu E, Nemes R, Chifiriuc MC. Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania. Rev. Chim.[internet]. 2018 Nov;69(11):3126-3132. Available from: https://doi.org/10.37358/RC.18.11.6697

APA 6th edition
Mitache, M.M., Banu, O., Borcan, E., Gheorghe, I., Marutescu, L., Popa, m.,... Chifiriuc, M.C. (2018). Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania. Revista de Chimie, 69(11), 3126-3132. https://doi.org/10.37358/RC.18.11.6697

Harvard
Mitache, M.M., Banu, O., Borcan, E., Gheorghe, I., Marutescu, L., Popa, m., Predescu, M., Rusu, E., Nemes, R., Chifiriuc, M.C. (2018). 'Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania', Revista de Chimie, 69(11), pp. 3126-3132. https://doi.org/10.37358/RC.18.11.6697

IEEE
M.M. Mitache, O. Banu, E. Borcan, I. Gheorghe, L. Marutescu, m. Popa, M. Predescu, E. Rusu, R. Nemes, M.C. Chifiriuc, "Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania". Revista de Chimie, vol. 69, no. 11, pp. 3126-3132, 2018. [online]. https://doi.org/10.37358/RC.18.11.6697

Text
Mihaela Magdalena Mitache, Otilia Banu, Elvira Borcan, Irina Gheorghe, Luminita Marutescu,marcela Popa, Miruna Predescu, Elena Rusu, Roxana Nemes, Mariana Carmen Chifiriuc,
Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania,
Revista de Chimie,
Volume 69, Issue 11,
2018,
Pages 3126-3132,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.11.6697.
(https://revistadechimie.ro/Articles.asp?ID=6697)
Keywords: resistance; virulence; nosocomial infections

RIS
TY - JOUR
T1 - Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania
A1 - Mitache, Mihaela Magdalena
A2 - Banu, Otilia
A3 - Borcan, Elvira
A4 - Gheorghe, Irina
A5 - Marutescu, Luminita
A6 - Popa, marcela
A7 - Predescu, Miruna
A8 - Rusu, Elena
A9 - Nemes, Roxana
A10 - Chifiriuc, Mariana Carmen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 11
SP - 3126
EP - 3132
UR - https://doi.org/10.37358/RC.18.11.6697
KW - resistance
KW - virulence
KW - nosocomial infections
ER -

BibTex
@article{RevCh2018P3126,
author = {Mitache Mihaela Magdalena and Banu Otilia and Borcan Elvira and Gheorghe Irina and Marutescu Luminita and Popa marcela and Predescu Miruna and Rusu Elena and Nemes Roxana and Chifiriuc Mariana Carmen},
title = {Resistance and Virulence Patterns in Gram Negative and Gram Positives Rods Isolated from the Hospital Environment in Bucharest, Romania},
journal = {Revista de Chimie},
volume = {69},
number = {11},
pages = {3126-3132},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.11.6697},
url = {https://revistadechimie.ro/Articles.asp?ID=6697}
}

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