Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria

Stoica, Catalina; Ancuta, Paul Nicolae; Lucaciu, Irina Eugenia; Banciu, Alina Roxana; Sorea, Sorin; Atanasescu, Anca; Lazar, Mihai Nita

Catalina Stoica, Paul Nicolae Ancuta, Irina Eugenia Lucaciu, Alina Roxana Banciu, Sorin Sorea, Anca Atanasescu, Mihai Nita Lazar

Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria

Abstract:

The waterborne pathogenic bacteria, especially the enteric bacteria of human fecal origin, have become currently a global public health issue. The detection and quantification of drinking water microorganisms have been an essential part of any quality control or water safety management plan interconnected to enteric bacterial pathogens such as Salmonella spp., Shigella spp., Vibrio cholerae or to non-faecal bacterial pathogens such as Pseudomonas aeruginosa. The standard methods of detecting waterborne pathogenic bacteria are time-consuming due to the bacterial growing step in a specific culture media, followed by isolation, microbiological and/or serological identiûcation and in some cases followed by subspeciûc characterization. This study aimed to develop a faster, powerful, more sensitive and reproducible diagnostic tool to monitor a specific pathogen contamination in drinking water (Pseudomonas aeruginosa) by specific antibody-antigen interactions followed by a computerized technology to identify bacteria as a digital image from water samples. Digital Image processing was carried out using National Instruments Vision Assistant Software. Pseudomonas aeruginosa was specifically detected by immunofluorescence technique with fluorophore tagged antibodies, and then the image formation in fluorescence microscopy was processed by computer vision software functions organized in pipeline-like data analysis processing. Overall, these techniques proved to be a reliable one, time-effective and sensitive for diagnosis and prevention of drinking water quality and waterborne bacterial disease.

Keywords:
antibody-antigen interaction; bacteria; immunofluorescence; high-tech technology

Issue: 2018, Volume 69, Issue 11

Pages: 3266-3270

Publication date: 2018/12/15

https://doi.org/10.37358/RC.18.11.6727

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
STOICA, C., ANCUTA, P.N., LUCACIU, I.E., BANCIU, A.R., SOREA, S., ATANASESCU, A., LAZAR, M.N., Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria, Rev. Chim., 69(11), 2018, 3266-3270.

Vancouver
Stoica C, Ancuta PN, Lucaciu IE, Banciu AR, Sorea S, Atanasescu A, Lazar MN. Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria. Rev. Chim.[internet]. 2018 Nov;69(11):3266-3270. Available from: https://doi.org/10.37358/RC.18.11.6727

APA 6th edition
Stoica, C., Ancuta, P.N., Lucaciu, I.E., Banciu, A.R., Sorea, S., Atanasescu, A. & Lazar, M.N. (2018). Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria. Revista de Chimie, 69(11), 3266-3270. https://doi.org/10.37358/RC.18.11.6727

Harvard
Stoica, C., Ancuta, P.N., Lucaciu, I.E., Banciu, A.R., Sorea, S., Atanasescu, A., Lazar, M.N. (2018). 'Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria', Revista de Chimie, 69(11), pp. 3266-3270. https://doi.org/10.37358/RC.18.11.6727

IEEE
C. Stoica, P.N. Ancuta, I.E. Lucaciu, A.R. Banciu, S. Sorea, A. Atanasescu, M.N. Lazar, "Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria". Revista de Chimie, vol. 69, no. 11, pp. 3266-3270, 2018. [online]. https://doi.org/10.37358/RC.18.11.6727

Text
Catalina Stoica, Paul Nicolae Ancuta, Irina Eugenia Lucaciu, Alina Roxana Banciu, Sorin Sorea, Anca Atanasescu, Mihai Nita Lazar,
Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria,
Revista de Chimie,
Volume 69, Issue 11,
2018,
Pages 3266-3270,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.11.6727.
(https://revistadechimie.ro/Articles.asp?ID=6727)
Keywords: antibody-antigen interaction; bacteria; immunofluorescence; high-tech technology

RIS
TY - JOUR
T1 - Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria
A1 - Stoica, Catalina
A2 - Ancuta, Paul Nicolae
A3 - Lucaciu, Irina Eugenia
A4 - Banciu, Alina Roxana
A5 - Sorea, Sorin
A6 - Atanasescu, Anca
A7 - Lazar, Mihai Nita
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 11
SP - 3266
EP - 3270
UR - https://doi.org/10.37358/RC.18.11.6727
KW - antibody-antigen interaction
KW - bacteria
KW - immunofluorescence
KW - high-tech technology
ER -

BibTex
@article{RevCh2018P3266,
author = {Stoica Catalina and Ancuta Paul Nicolae and Lucaciu Irina Eugenia and Banciu Alina Roxana and Sorea Sorin and Atanasescu Anca and Lazar Mihai Nita},
title = {Computerized High-tech Detection Technology of Immunofluorescence Labelled Waterborne Pathogenic Bacteria},
journal = {Revista de Chimie},
volume = {69},
number = {11},
pages = {3266-3270},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.11.6727},
url = {https://revistadechimie.ro/Articles.asp?ID=6727}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)