Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model

Hussein, Hussam Al; Harpa, Marius; Movileanu, Ionela; Hussein, Hamida Al; Suciu, Horatiu; Branzaniuc, Klara; Simionescu, Dan

Hussam Al Hussein, Marius Harpa, Ionela Movileanu, Hamida Al Hussein, Horatiu Suciu, Klara Branzaniuc, Dan Simionescu

Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model

Abstract:

Many methods of stem cells collection and isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. The present study purposed to describe a minimally invasive surgical protocol for adipose tissue collection from sheep’s inter-scapular area. The procedure was carried out on adult sheeps, in aseptic conditions. A light sedation protocol with Detomidine was performed, the recovery from anesthesia being carried out with Atipamezole. Throughout the sedation, the surgical procedure and the recovery from anesthesia, the vital functions of the animal were monitored. The adipose tissue samples collected in sterile tubes with culture medium (Dulbecco’s modified Eagle’s medium - DMEM/10% / FBS10 - fetal bovine serum, 2% antibiotic/antifungal), have been succesfully used by our research team for adipose tissue derived stem cells (ADSCs) isolation for further use in cardiac valves tissue engineering.

Keywords:
adipose tissue; sheep; stem cells; Detomidine; Atipamezole

Issue: 2019, Volume 70, Issue 5

Pages: 1826-1828

Publication date: 2019/6/15

https://doi.org/10.37358/RC.19.5.7224

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
HUSSEIN, H.A., HARPA, M., MOVILEANU, I., HUSSEIN, H.A., SUCIU, H., BRANZANIUC, K., SIMIONESCU, D., Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model, Rev. Chim., 70(5), 2019, 1826-1828.

Vancouver
Hussein HA, Harpa M, Movileanu I, Hussein HA, Suciu H, Branzaniuc K, Simionescu D. Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model. Rev. Chim.[internet]. 2019 May;70(5):1826-1828. Available from: https://doi.org/10.37358/RC.19.5.7224

APA 6th edition
Hussein, H.A., Harpa, M., Movileanu, I., Hussein, H.A., Suciu, H., Branzaniuc, K. & Simionescu, D. (2019). Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model. Revista de Chimie, 70(5), 1826-1828. https://doi.org/10.37358/RC.19.5.7224

Harvard
Hussein, H.A., Harpa, M., Movileanu, I., Hussein, H.A., Suciu, H., Branzaniuc, K., Simionescu, D. (2019). 'Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model', Revista de Chimie, 70(5), pp. 1826-1828. https://doi.org/10.37358/RC.19.5.7224

IEEE
H.A. Hussein, M. Harpa, I. Movileanu, H.A. Hussein, H. Suciu, K. Branzaniuc, D. Simionescu, "Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model". Revista de Chimie, vol. 70, no. 5, pp. 1826-1828, 2019. [online]. https://doi.org/10.37358/RC.19.5.7224

Text
Hussam Al Hussein, Marius Harpa, Ionela Movileanu, Hamida Al Hussein, Horatiu Suciu, Klara Branzaniuc, Dan Simionescu,
Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model,
Revista de Chimie,
Volume 70, Issue 5,
2019,
Pages 1826-1828,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.5.7224.
(https://revistadechimie.ro/Articles.asp?ID=7224)
Keywords: adipose tissue; sheep; stem cells; Detomidine; Atipamezole

RIS
TY - JOUR
T1 - Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model
A1 - Hussein, Hussam Al
A2 - Harpa, Marius
A3 - Movileanu, Ionela
A4 - Hussein, Hamida Al
A5 - Suciu, Horatiu
A6 - Branzaniuc, Klara
A7 - Simionescu, Dan
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 5
SP - 1826
EP - 1828
UR - https://doi.org/10.37358/RC.19.5.7224
KW - adipose tissue
KW - sheep
KW - stem cells
KW - Detomidine
KW - Atipamezole
ER -

BibTex
@article{RevCh2019P1826,
author = {Hussein Hussam Al and Harpa Marius and Movileanu Ionela and Hussein Hamida Al and Suciu Horatiu and Branzaniuc Klara and Simionescu Dan},
title = {Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model},
journal = {Revista de Chimie},
volume = {70},
number = {5},
pages = {1826-1828},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.5.7224},
url = {https://revistadechimie.ro/Articles.asp?ID=7224}
}

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