In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture

Maria, Gheorghe; Gijiu, Cristiana Luminita; Cebanu, Iulian; Maria, Cristina; Tociu, Carmen

Gheorghe Maria, Cristiana Luminita Gijiu, Iulian Cebanu, Cristina Maria, Carmen Tociu

In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture

Abstract:

Bioreactor optimization is a common engineering problem difficult to be solved due to the large number of influential variables of high variability. Production of monoclonal antibody is a well-known method to synthesize a large number of identical antibodies (that is of uniform characteristics, also called monoclonal antibodies, mAb). Due to such reasons intense efforts have been invested to maximize the production of mAb by using hybridoma cell culture. Based on an adequate kinetic model from literature (experimentally checked) this paper focus on pointing-out the major role of the net evolution of the viable biomass (growth, and decay) in the location of the optimal operating setpoint (SP) of a three-phase mechanically agitated batch bioreactor (TPMAB) with immobilized hybridoma culture. This in-silico analysis opens the possibility I) to optimize the bioreactor performances by placing the SP in the most favourable location, by adjusting the substrate and biomass initial load in the bioreactor according to the preliminary determined characteristics of a modified / improved biomass; ii) to optimize the batch-to-batch operation mode (not approached here) according to the time-varying characteristics of the biomass culture, or iii) to determine the optimal operation of the bioreactor in a fed-batch operating mode (not approached here).

Keywords:
monoclonal antibodies; hybridoma cell culture; batch bioreactor optimization; biomass evolution dynamic simulation

Issue: 2019, Volume 70, Issue 8

Pages: 2985-2992

Publication date: 2019/9/15

https://doi.org/10.37358/RC.19.8.7470

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MARIA, G., GIJIU, C.L., CEBANU, I., MARIA, C., TOCIU, C., In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture, Rev. Chim., 70(8), 2019, 2985-2992.

Vancouver
Maria G, Gijiu CL, Cebanu I, Maria C, Tociu C. In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture. Rev. Chim.[internet]. 2019 Aug;70(8):2985-2992. Available from: https://doi.org/10.37358/RC.19.8.7470

APA 6th edition
Maria, G., Gijiu, C.L., Cebanu, I., Maria, C. & Tociu, C. (2019). In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture. Revista de Chimie, 70(8), 2985-2992. https://doi.org/10.37358/RC.19.8.7470

Harvard
Maria, G., Gijiu, C.L., Cebanu, I., Maria, C., Tociu, C. (2019). 'In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture', Revista de Chimie, 70(8), pp. 2985-2992. https://doi.org/10.37358/RC.19.8.7470

IEEE
G. Maria, C.L. Gijiu, I. Cebanu, C. Maria, C. Tociu, "In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture". Revista de Chimie, vol. 70, no. 8, pp. 2985-2992, 2019. [online]. https://doi.org/10.37358/RC.19.8.7470

Text
Gheorghe Maria, Cristiana Luminita Gijiu, Iulian Cebanu, Cristina Maria, Carmen Tociu,
In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture,
Revista de Chimie,
Volume 70, Issue 8,
2019,
Pages 2985-2992,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.8.7470.
(https://revistadechimie.ro/Articles.asp?ID=7470)
Keywords: monoclonal antibodies; hybridoma cell culture; batch bioreactor optimization; biomass evolution dynamic simulation

RIS
TY - JOUR
T1 - In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture
A1 - Maria, Gheorghe
A2 - Gijiu, Cristiana Luminita
A3 - Cebanu, Iulian
A4 - Maria, Cristina
A5 - Tociu, Carmen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 8
SP - 2985
EP - 2992
UR - https://doi.org/10.37358/RC.19.8.7470
KW - monoclonal antibodies; hybridoma cell culture; batch bioreactor optimization; biomass evolution dynamic simulation
ER -

BibTex
@article{RevCh2019P2985,
author = {Maria Gheorghe and Gijiu Cristiana Luminita and Cebanu Iulian and Maria Cristina and Tociu Carmen},
title = {In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture},
journal = {Revista de Chimie},
volume = {70},
number = {8},
pages = {2985-2992},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.8.7470},
url = {https://revistadechimie.ro/Articles.asp?ID=7470}
}

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