Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates

Hamid, Mohammad I. Abd El; Fatah, Walaa M. Abd El; Morsi, Adel A. El; Draz, Mohamed Shehata; Kallakuri, Shantanu; Bungau, Simona; Endres, Laura; Bardaca (Urducea), Cristina; Hafez, Elsayed E.

Mohammad I. Abd El Hamid, Walaa M. Abd El Fatah, Adel A. El Morsi, Mohamed Shehata Draz, Shantanu Kallakuri, Simona Bungau, Laura Endres, Cristina Bardaca (Urducea), Elsayed E. Hafez

Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates

Abstract:

The control of the viral infections requires efficient, rapid and safe testing procedures. We tested the efficiency of a quick and reliable alternative assay to the standard plaque assay (PA) in the detection of viral inhibition. Using MS-2 and FX-174 phages, the developed assay was used to evaluate the potential of cyanobacterial phycobiliproteins (PBS) as natural antiviral bioactive molecules against HIV and HCV. The antiviral activity was examined by mixing the phage with crude aqueous extracts in a 1:1 (v/v) ratio for 1hr at 4oC. The viral titer of MS-2 and FX-174 was reduced by 0.92 LR and 1.19 LR, respectively. In the case of the crude PBS extract of Spirulina platensis, similar result trends were obtained by virion titration which indicated that a strong correlation between the two assays was verified (³90 %). The morphological characteristics of both phages were severely affected when treated with the extract, whereas the characteristic features of their hosts seem unaffected. The PBS was found to affect the adsorption of the phages on their bacterial hosts and consequently viral reproduction. In conclusion, MS-2 and FX-174 can be used in drug evaluation by the well standardized method developed in this study.

Keywords:
Antiviral assay; surrogates; MS-2; FX-174; phycobiliproteins; cyanobacteria; virion titration

Issue: 2019, Volume 70, Issue 9

Pages: 3115-3122

Publication date: 2019/10/15

https://doi.org/10.37358/RC.19.9.7499

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
HAMID, M.I.A.E., FATAH, W.M.A.E., MORSI, A.A.E., DRAZ, M.S., KALLAKURI, S., BUNGAU, S., ENDRES, L., BARDACA (URDUCEA), C., HAFEZ, E.E., Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates, Rev. Chim., 70(9), 2019, 3115-3122.

Vancouver
Hamid MIAE, Fatah WMAE, Morsi AAE, Draz MS, Kallakuri S, Bungau S, Endres L, Bardaca (Urducea) C, Hafez EE. Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates. Rev. Chim.[internet]. 2019 Sep;70(9):3115-3122. Available from: https://doi.org/10.37358/RC.19.9.7499

APA 6th edition
Hamid, M.I.A.E., Fatah, W.M.A.E., Morsi, A.A.E., Draz, M.S., Kallakuri, S., Bungau, S.,... Hafez, E.E. (2019). Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates. Revista de Chimie, 70(9), 3115-3122. https://doi.org/10.37358/RC.19.9.7499

Harvard
Hamid, M.I.A.E., Fatah, W.M.A.E., Morsi, A.A.E., Draz, M.S., Kallakuri, S., Bungau, S., Endres, L., Bardaca (Urducea), C., Hafez, E.E. (2019). 'Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates', Revista de Chimie, 70(9), pp. 3115-3122. https://doi.org/10.37358/RC.19.9.7499

IEEE
M.I.A.E. Hamid, W.M.A.E. Fatah, A.A.E. Morsi, M.S. Draz, S. Kallakuri, S. Bungau, L. Endres, C. Bardaca(Urducea), E.E. Hafez, "Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates". Revista de Chimie, vol. 70, no. 9, pp. 3115-3122, 2019. [online]. https://doi.org/10.37358/RC.19.9.7499

Text
Mohammad I. Abd El Hamid, Walaa M. Abd El Fatah, Adel A. El Morsi, Mohamed Shehata Draz, Shantanu Kallakuri, Simona Bungau, Laura Endres, Cristina Bardaca(Urducea), Elsayed E. Hafez,
Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates,
Revista de Chimie,
Volume 70, Issue 9,
2019,
Pages 3115-3122,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.9.7499.
(https://revistadechimie.ro/Articles.asp?ID=7499)
Keywords: Antiviral assay; surrogates; MS-2; FX-174; phycobiliproteins; cyanobacteria; virion titration

RIS
TY - JOUR
T1 - Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates
A1 - Hamid, Mohammad I. Abd El
A2 - Fatah, Walaa M. Abd El
A3 - Morsi, Adel A. El
A4 - Draz, Mohamed Shehata
A5 - Kallakuri, Shantanu
A6 - Bungau, Simona
A7 - Endres, Laura
A8 - Bardaca (Urducea), Cristina
A9 - Hafez, Elsayed E.
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 9
SP - 3115
EP - 3122
UR - https://doi.org/10.37358/RC.19.9.7499
KW - Antiviral assay
KW - surrogates
KW - MS-2
KW - FX-174
KW - phycobiliproteins
KW - cyanobacteria
KW - virion titration
ER -

BibTex
@article{RevCh2019P3115,
author = {Hamid Mohammad I. Abd El and Fatah Walaa M. Abd El and Morsi Adel A. El and Draz Mohamed Shehata and Kallakuri Shantanu and Bungau Simona and Endres Laura and Bardaca (Urducea) Cristina and Hafez Elsayed E.},
title = {Anti-HIV / HCV Activity of Cyanobacterial Phycobiliproteins by a New Standardized Method Using Bacteriophage Surrogates},
journal = {Revista de Chimie},
volume = {70},
number = {9},
pages = {3115-3122},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.9.7499},
url = {https://revistadechimie.ro/Articles.asp?ID=7499}
}

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