Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli

Bodor, Zsolt; Lanyi, Szabolcs; Albert, Beata; Bodor, Katalin; Nechifor, Aurelia Cristina; Miklossy, Ildiko

Zsolt Bodor, Szabolcs Lanyi, Beata Albert, Katalin Bodor, Aurelia Cristina Nechifor, Ildiko Miklossy

Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli

Abstract:

Bio-based, environmentally benign production of commodity chemicals such as 1,4-butanediol (BDO) from renewable feedstocks is highly challenging due to the lack of natural synthesis pathways. Herein, we present a systematic model-driven evaluation of the production potential for Escherichia coli to produce BDO from renewable carbohydrates (glucose, glycerol). Computational analysis was carried out in order to decipher the metabolic characteristics under various genetic and environmental conditions. Optimal strain designs were achieved using only two (adhE2- alcohol dehydrogenase and cat/sucCD- 4-hydroxybutyrate-CoA transferase/4-hydroxybutyryl-CoA ligase) heterologous reactions; highest yields were attained for: glucose ~0.37 g g-1 (3 knockouts, anaerobically) and glycerol ~0.43 g g-1 (4 knockouts, microaerobically). The maximum achievable production yield was over 95% of the theoretical maximum potential for glucose and over 75% for glycerol. In regards to the genome-scale metabolic model predictions, a metabolically engineered E. coli was created to analyze the new biosynthetic pathway stability and functionality. Considering the preliminary outcomes the strain and pathway is stable under fermentative conditions and a limited quantity of BDO ~1 mg L-1 was obtained, therefore long-term adaptive evolution is mandatory. This study outlines a strain design and analysis pipeline -systems biology-based approach- for non-native compounds production strains.

Keywords:
renewable feedstocks; bioconversion; 1;4-butanediol; COBRA; E. coli; strain design

Issue: 2019, Volume 70, Issue 11

Pages: 3808-3817

Publication date: 2019/12/15

https://doi.org/10.37358/RC.70.19.11.7651

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BODOR, Z., LANYI, S., ALBERT, B., BODOR, K., NECHIFOR, A.C., MIKLOSSY, I., Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli, Rev. Chim., 70(11), 2019, 3808-3817.

Vancouver
Bodor Z, Lanyi S, Albert B, Bodor K, Nechifor AC, Miklossy I. Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli. Rev. Chim.[internet]. 2019 Nov;70(11):3808-3817. Available from: https://doi.org/10.37358/RC.70.19.11.7651

APA 6th edition
Bodor, Z., Lanyi, S., Albert, B., Bodor, K., Nechifor, A.C. & Miklossy, I. (2019). Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli. Revista de Chimie, 70(11), 3808-3817. https://doi.org/10.37358/RC.70.19.11.7651

Harvard
Bodor, Z., Lanyi, S., Albert, B., Bodor, K., Nechifor, A.C., Miklossy, I. (2019). 'Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli', Revista de Chimie, 70(11), pp. 3808-3817. https://doi.org/10.37358/RC.70.19.11.7651

IEEE
Z. Bodor, S. Lanyi, B. Albert, K. Bodor, A.C. Nechifor, I. Miklossy, "Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli". Revista de Chimie, vol. 70, no. 11, pp. 3808-3817, 2019. [online]. https://doi.org/10.37358/RC.70.19.11.7651

Text
Zsolt Bodor, Szabolcs Lanyi, Beata Albert, Katalin Bodor, Aurelia Cristina Nechifor, Ildiko Miklossy,
Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli,
Revista de Chimie,
Volume 70, Issue 11,
2019,
Pages 3808-3817,
ISSN 2668-8212,
https://doi.org/10.37358/RC.70.19.11.7651.
(https://revistadechimie.ro/Articles.asp?ID=7651)
Keywords: renewable feedstocks; bioconversion; 1;4-butanediol; COBRA; E. coli; strain design

RIS
TY - JOUR
T1 - Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli
A1 - Bodor, Zsolt
A2 - Lanyi, Szabolcs
A3 - Albert, Beata
A4 - Bodor, Katalin
A5 - Nechifor, Aurelia Cristina
A6 - Miklossy, Ildiko
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 11
SP - 3808
EP - 3817
UR - https://doi.org/10.37358/RC.70.19.11.7651
KW - renewable feedstocks; bioconversion; 1
KW - 4-butanediol; COBRA
KW - E. coli
KW - strain design
ER -

BibTex
@article{RevCh2019P3808,
author = {Bodor Zsolt and Lanyi Szabolcs and Albert Beata and Bodor Katalin and Nechifor Aurelia Cristina and Miklossy Ildiko},
title = {Model Driven Analysis of the Biosynthesis of 1,4-butanediol from Renewable Feedstocks in Escherichia coli},
journal = {Revista de Chimie},
volume = {70},
number = {11},
pages = {3808-3817},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.70.19.11.7651},
url = {https://revistadechimie.ro/Articles.asp?ID=7651}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)