Process Identification and Model-Based Operating Policies for an Enzymatic Fed-Batch Reactor of Rare Sugar Production

Process Identification and Model-Based Operating Policies for an Enzymatic Fed-Batch Reactor of Rare Sugar Production

Abstract:

Model-based optimization of fed-batch bioreactors is an intensively studied problem due to the potential economic benefits and increased control possibilities revealed by the (sub)optimal operating policies. However, the bioprocess kinetics high complexity, the nonlinear process dynamics including a wide range of time constants, the small number of recorded variables of variate observability, the data low reproducibility, bioprocess particular sensitivity to operating conditions, inhibitors, limiting steps, and technological constraints, all these lead to difficulties in identifying the process, in deriving a feasible optimal operating policy, and in result generalization. The constrained dynamic optimization problem, accounting for substrate set-point concentrations, feeding time-step patterns, feeding characteristics, temperature, and pH, is difficult to be solved due to the existing multiple feasible optima. Various techniques have been proposed, including continuous and/or discrete variable formulations, and a large variety of optimization procedures. To quickly derive sub-optimal operating policies for a fed-batch enzymatic reactor, a three-steps procedure is checked in this paper: process kinetics identification based on separate experiments and mechanistic studies; process sensitivity analysis vs. operating conditions; model-based prediction of sub-optimal operating policies involving periodic enzyme injections adapted to system dynamics. The approached process, i.e. the enzymatic oxidation of D-glucose, belongs to rare sugar (keto-derivatives) production from saccharides. The process and reactor present some particularities, i.e. a limited oxygenation rate, an enzyme deactivation due to one of the products and some of the impurities (as iron traces), and a complex and partly known reaction mechanism. The obtained periodic feeding policies, based on a reduced process / reactor model, are easy to be implemented, and recommended as an approximate route for improving the bioreactor performance. Keywords: fed-batch bioreactor, optimal operation, rare sugar production, 2-keto-D-glucose

Issue: 2005, Volume 56, Issue 11

Pages:

This article is published under the Creative Commons Attribution 4.0 International License

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