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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2020, Volume 71, Issue 1, 39-44

https://doi.org/10.37358/RC.20.1.7808

Oana Alexandra Patrautanu, Oana Teodora Ciuperca, Valentin I. Popa, Irina Volf

Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods

Abstract:

This work assessed the qualitative and quantitative polyphenols profile in a crude ethanol extract separated from spruce bark (two fractions with [0.25 and 1 mm diameter) using ultrasound assisted extraction (UAE) as a green extraction method. The evaluation of phenolic acids and condensed tannins profile was performed using instrumental (UV-VIS spectrometry), qualitative (Thin Layer Chromatography, TLC) and quantitative (HPTLC densitometry) methods. Using ultrasound assisted extraction (UAE) technique under specific parameters, the higher total phenolic content (TPC) was 29.785 mg GAE/g-1 for spruce bark fraction with particle size [0.25 mm and 14.448 mg GAE/g-1 for fraction with 1 mm diameters. The TLC assay of the crude ethanol extract was performed considering the standards: gallic, sinapic, p-coumaric and vanillic acids, catechin, epicatechin and tannic acid. Smaller material particle sizes lead to higher yield of polyphenols in ethanol extract. The quantitative evaluation by HPTLC densitometry revealed following amounts: sinapic acid 0.84 mg/g, p-coumaric acid 0.61 mg/g, catechin 1.03 mg/g, tannic acid 2.81 mg/g. Considering the chemical composition, the spread and availability, spruce bark could be considered as a resource with environmental and economic benefits.
Keywords:
spruce bark; ultrasounds assisted extraction; polyphenols; phytochemical screening; HPTLC (High Performance Thin Layer Chromatography)

Issue: 2020, Volume 71, Issue 1
Pages: 39-44
Publication date: 2020/2/7
https://doi.org/10.37358/RC.20.1.7808
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
PATRAUTANU, O.A., CIUPERCA, O.T., POPA, V.I., VOLF, I., Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods, Rev. Chim., 71(1), 2020, 39-44.

Vancouver
Patrautanu OA, Ciuperca OT, Popa VI, Volf I. Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods. Rev. Chim.[internet]. 2020 Jan;71(1):39-44. Available from: https://doi.org/10.37358/RC.20.1.7808


APA 6th edition
Patrautanu, O.A., Ciuperca, O.T., Popa, V.I. & Volf, I. (2020). Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods. Revista de Chimie, 71(1), 39-44. https://doi.org/10.37358/RC.20.1.7808


Harvard
Patrautanu, O.A., Ciuperca, O.T., Popa, V.I., Volf, I. (2020). 'Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods', Revista de Chimie, 71(1), pp. 39-44. https://doi.org/10.37358/RC.20.1.7808


IEEE
O.A. Patrautanu, O.T. Ciuperca, V.I. Popa, I. Volf, "Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods". Revista de Chimie, vol. 71, no. 1, pp. 39-44, 2020. [online]. https://doi.org/10.37358/RC.20.1.7808


Text
Oana Alexandra Patrautanu, Oana Teodora Ciuperca, Valentin I. Popa, Irina Volf,
Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods,
Revista de Chimie,
Volume 71, Issue 1,
2020,
Pages 39-44,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.1.7808.
(https://revistadechimie.ro/Articles.asp?ID=7808)
Keywords: spruce bark; ultrasounds assisted extraction; polyphenols; phytochemical screening; HPTLC (High Performance Thin Layer Chromatography)


RIS
TY - JOUR
T1 - Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods
A1 - Patrautanu, Oana Alexandra
A2 - Ciuperca, Oana Teodora
A3 - Popa, Valentin I.
A4 - Volf, Irina
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 1
SP - 39
EP - 44
UR - https://doi.org/10.37358/RC.20.1.7808
KW - spruce bark
KW - ultrasounds assisted extraction
KW - polyphenols
KW - phytochemical screening
KW - HPTLC (High Performance Thin Layer Chromatography)
ER -


BibTex
@article{RevCh2020P39,
author = {Patrautanu Oana Alexandra and Ciuperca Oana Teodora and Popa Valentin I. and Volf Irina},
title = {Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods},
journal = {Revista de Chimie},
volume = {71},
number = {1},
pages = {39-44},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.1.7808},
url = {https://revistadechimie.ro/Articles.asp?ID=7808}
}
 
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