Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC

Catana, Luminita; Catana, Monica; Iorga, Enuta; Asanica, Adrian Constantin; Lazar, Monica-Alexandra; Lazar, Anda-Gratiela; Belc, Nastasia; Pirvu, Gina

Luminita Catana, Monica Catana, Enuta Iorga, Adrian Constantin Asanica, Monica-Alexandra Lazar, Anda-Gratiela Lazar, Nastasia Belc, Gina Pirvu

Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC

Abstract:

An analytical method was developed and validated for separation, detection and quantification of carotenoids (all-trans lutein, β-carotene and all-trans lycopene) in tomato waste powder by high-performance liquid chromatography (HPLC-DAD). Extraction of carotenoids was achieved in acetone under nitrogen atmosphere and magnetic stirring. Carotenoids were separated on a reverse-phase C30, 3 μm column (250 ×4.6 mm) coupled to a 20 × 4.6 mm C30 guard column using mobile phases consisting of (A) methanol/ water (98:2, v/v), (B) methanol/water (95:5, v/v) and (C) methyl tert-butyl ether.The method has a good sensitivity (LOD = 0.161 -0.333 μg/mL and LOQ = 0.484 - 1.000 μg/mL) and a good precision (RDS (r) = 0.67 - 1.15% for injection repeatability; RSD (r) = 1.02 - 2.14% for analysis repeatability intra-day; RSD (r) = 1.23 - 2.43% for intermediate precision; RSD (R) = 1.57 - 3.07 % for intra-laboratory reproducibility. The method was applied byanalyzing 8 tomato waste powders, obtained through tomatoes processing as juice. Their carotenoids content varied in the following ranges: 1.474 - 2.452 mg/100g for all-trans lutein; 9.645 - 11.587 mg/100g for β-carotene; 60.150 - 64.855 mg/100g for all-trans lycopene.

Keywords:
carotenoids; validation; HPLC-DAD; powder; tomato waste

Issue: 2020, Volume 71, Issue 1

Pages: 342-349

Publication date: 2020/2/7

https://doi.org/10.37358/RC.20.1.7855

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
CATANA, L., CATANA, M., IORGA, E., ASANICA, A.C., LAZAR, M., LAZAR, A., BELC, N., PIRVU, G., Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC, Rev. Chim., 71(1), 2020, 342-349.

Vancouver
Catana L, Catana M, Iorga E, Asanica AC, Lazar M, Lazar A, Belc N, Pirvu G. Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC. Rev. Chim.[internet]. 2020 Jan;71(1):342-349. Available from: https://doi.org/10.37358/RC.20.1.7855

APA 6th edition
Catana, L., Catana, M., Iorga, E., Asanica, A.C., Lazar, M., Lazar, A., Belc, N. & Pirvu, G. (2020). Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC. Revista de Chimie, 71(1), 342-349. https://doi.org/10.37358/RC.20.1.7855

Harvard
Catana, L., Catana, M., Iorga, E., Asanica, A.C., Lazar, M., Lazar, A., Belc, N., Pirvu, G. (2020). 'Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC', Revista de Chimie, 71(1), pp. 342-349. https://doi.org/10.37358/RC.20.1.7855

IEEE
L. Catana, M. Catana, E. Iorga, A.C. Asanica, M. Lazar, A. Lazar, N. Belc, G. Pirvu, "Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC". Revista de Chimie, vol. 71, no. 1, pp. 342-349, 2020. [online]. https://doi.org/10.37358/RC.20.1.7855

Text
Luminita Catana, Monica Catana, Enuta Iorga, Adrian Constantin Asanica, Monica-Alexandra Lazar, Anda-Gratiela Lazar, Nastasia Belc, Gina Pirvu,
Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC,
Revista de Chimie,
Volume 71, Issue 1,
2020,
Pages 342-349,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.1.7855.
(https://revistadechimie.ro/Articles.asp?ID=7855)
Keywords: carotenoids; validation; HPLC-DAD; powder; tomato waste

RIS
TY - JOUR
T1 - Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC
A1 - Catana, Luminita
A2 - Catana, Monica
A3 - Iorga, Enuta
A4 - Asanica, Adrian Constantin
A5 - Lazar, Monica-Alexandra
A6 - Lazar, Anda-Gratiela
A7 - Belc, Nastasia
A8 - Pirvu, Gina
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 1
SP - 342
EP - 349
UR - https://doi.org/10.37358/RC.20.1.7855
KW - carotenoids
KW - validation
KW - HPLC-DAD
KW - powder
KW - tomato waste
ER -

BibTex
@article{RevCh2020P342,
author = {Catana Luminita and Catana Monica and Iorga Enuta and Asanica Adrian Constantin and Lazar Monica-Alexandra and Lazar Anda-Gratiela and Belc Nastasia and Pirvu Gina},
title = {Internal Validation of Rapid and Performant Method for Carotenoids Determination in Tomato Waste Powder by HPLC},
journal = {Revista de Chimie},
volume = {71},
number = {1},
pages = {342-349},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.1.7855},
url = {https://revistadechimie.ro/Articles.asp?ID=7855}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)