Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children

Belei, Oana; Iacob, Emil Radu; Iacob, Daniela; Amaricai, Elena; Marginean, Otilia

Oana Belei, Emil Radu Iacob, Daniela Iacob, Elena Amaricai, Otilia Marginean

Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children

Abstract:

Recent describing of atypical, silent and latent form of celiac disease (CD) increased the preocupation for screening methods.To perform a comparative study of immunoglobulin A (IgA) anti tissue-transglutaminase (tTG) antibodies (Ab) assessment using chemiluminescence versus Enzyme Linked Immunosorbent Assay (ELISA).The study included two lots.The first lot consisted in 35 biopsy confirmed CD children aged between two and 18 years.The control lot included 40 children with normal duodenal morphology on intestinal biopsy that underwent upper digestive endoscopy for different gastrointestinal symptoms.Serum samples were provided from all subjects for IgA measurement, IgA anti tTG and EMA assessment. Immulite 2500 Anti tTG IgA (Siemens Co, LA, USA) and ImmuLisa anti-hu tTG antibody IgA ELISA (Immco Diagnostics Inc, NY, USA) kits were used for tTG-Ab assessment. All children underwent HLA typing for DQ2/DQ8. The sensitivity for IgA tTG assessment was greater for chemiluminescence (93,3%) versus ELISA (86,6%), p[0,05, while specificity, positive and negative predictive value didn’t register significant differences. Statistical comparison of the two tested methods revealed a better sensitivity for chemiluminescence. Diagnosis algorithm optimisation may be obtained by associating IgA anti tTG-Ab assessment using chemiluminescence followed by EMA confirmation by indirect immunofluorescence for CD screening.

Keywords:
celiac disease; children; tisue-transglutaminase; chemiluminescence; ELISA

Issue: 2020, Volume 71, Issue 2

Pages: 45-51

Publication date: 2020/3/3

https://doi.org/10.37358/RC.20.2.7890

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BELEI, O., IACOB, E.R., IACOB, D., AMARICAI, E., MARGINEAN, O., Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children, Rev. Chim., 71(2), 2020, 45-51.

Vancouver
Belei O, Iacob ER, Iacob D, Amaricai E, Marginean O. Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children. Rev. Chim.[internet]. 2020 Feb;71(2):45-51. Available from: https://doi.org/10.37358/RC.20.2.7890

APA 6th edition
Belei, O., Iacob, E.R., Iacob, D., Amaricai, E. & Marginean, O. (2020). Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children. Revista de Chimie, 71(2), 45-51. https://doi.org/10.37358/RC.20.2.7890

Harvard
Belei, O., Iacob, E.R., Iacob, D., Amaricai, E., Marginean, O. (2020). 'Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children', Revista de Chimie, 71(2), pp. 45-51. https://doi.org/10.37358/RC.20.2.7890

IEEE
O. Belei, E.R. Iacob, D. Iacob, E. Amaricai, O. Marginean, "Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children". Revista de Chimie, vol. 71, no. 2, pp. 45-51, 2020. [online]. https://doi.org/10.37358/RC.20.2.7890

Text
Oana Belei, Emil Radu Iacob, Daniela Iacob, Elena Amaricai, Otilia Marginean,
Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children,
Revista de Chimie,
Volume 71, Issue 2,
2020,
Pages 45-51,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.2.7890.
(https://revistadechimie.ro/Articles.asp?ID=7890)
Keywords: celiac disease; children; tisue-transglutaminase; chemiluminescence; ELISA

RIS
TY - JOUR
T1 - Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children
A1 - Belei, Oana
A2 - Iacob, Emil Radu
A3 - Iacob, Daniela
A4 - Amaricai, Elena
A5 - Marginean, Otilia
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 2
SP - 45
EP - 51
UR - https://doi.org/10.37358/RC.20.2.7890
KW - celiac disease
KW - children
KW - tisue-transglutaminase
KW - chemiluminescence
KW - ELISA
ER -

BibTex
@article{RevCh2020P45,
author = {Belei Oana and Iacob Emil Radu and Iacob Daniela and Amaricai Elena and Marginean Otilia},
title = {Chemiluminescent Immunoassay versus Enzyme Linked Immunosorbent Assays for IgA Anti-Tissue Transglutaminase Antibodies Assessment in Celiac Disease Children},
journal = {Revista de Chimie},
volume = {71},
number = {2},
pages = {45-51},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.2.7890},
url = {https://revistadechimie.ro/Articles.asp?ID=7890}
}

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