In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line

Baciu, Dora Domnica; Salageanu, Aurora; Visan, Teodor

Dora Domnica Baciu, Aurora Salageanu, Teodor Visan

In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line

Abstract:

Monosodium glutamate (E-621, abbreviated MSG) is a food additive widely used in the food domain as a flavor and taste enhancer. The present study aims to evaluate in vitro the effects of increased concentrations of MSG, using the RAW 264.7 murine macrophage line. The study was conducted in 3 complementary directions: first, establishing the ability of monosodium glutamate to induce cell mortality, by using the MTT assay to determine cell viability; second, establishing by its oxidizing activity if MSG is toxic for cells inducing oxidative stress, measured by the Griess test for determination of extracellular NO; third, to observe whether MSG presence induces an immune response quantified by the secretion of proinflammatory cytokines, TNFα in this case, measured through the immunoenzymatic ELISA technique.

Keywords:
monosodium glutamate; viability; cytotoxicity; proinflammatory effect; TNFα

Issue: 2020, Volume 71, Issue 2

Pages: 443-448

Publication date: 2020/3/3

https://doi.org/10.37358/RC.20.2.7947

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BACIU, D.D., SALAGEANU, A., VISAN, T., In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line, Rev. Chim., 71(2), 2020, 443-448.

Vancouver
Baciu DD, Salageanu A, Visan T. In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line. Rev. Chim.[internet]. 2020 Feb;71(2):443-448. Available from: https://doi.org/10.37358/RC.20.2.7947

APA 6th edition
Baciu, D.D., Salageanu, A. & Visan, T. (2020). In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line. Revista de Chimie, 71(2), 443-448. https://doi.org/10.37358/RC.20.2.7947

Harvard
Baciu, D.D., Salageanu, A., Visan, T. (2020). 'In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line', Revista de Chimie, 71(2), pp. 443-448. https://doi.org/10.37358/RC.20.2.7947

IEEE
D.D. Baciu, A. Salageanu, T. Visan, "In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line". Revista de Chimie, vol. 71, no. 2, pp. 443-448, 2020. [online]. https://doi.org/10.37358/RC.20.2.7947

Text
Dora Domnica Baciu, Aurora Salageanu, Teodor Visan,
In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line,
Revista de Chimie,
Volume 71, Issue 2,
2020,
Pages 443-448,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.2.7947.
(https://revistadechimie.ro/Articles.asp?ID=7947)
Keywords: monosodium glutamate; viability; cytotoxicity; proinflammatory effect; TNFα

RIS
TY - JOUR
T1 - In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line
A1 - Baciu, Dora Domnica
A2 - Salageanu, Aurora
A3 - Visan, Teodor
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 2
SP - 443
EP - 448
UR - https://doi.org/10.37358/RC.20.2.7947
KW - monosodium glutamate
KW - viability
KW - cytotoxicity
KW - proinflammatory effect
KW - TNFα
ER -

BibTex
@article{RevCh2020P443,
author = {Baciu Dora Domnica and Salageanu Aurora and Visan Teodor},
title = {In vitro Evaluation of the Antiproliferative Effect, Cytotoxicity and Proinflammatory Activity of the Food Additive Monosodium Glutamate on RAW 264.7 Cell Line},
journal = {Revista de Chimie},
volume = {71},
number = {2},
pages = {443-448},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.2.7947},
url = {https://revistadechimie.ro/Articles.asp?ID=7947}
}

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