Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search

Movileanu, Ionela; Brinzaniuc, Klara; Harpa, Marius M.; Cotoi, Ovidiu S.; Preda, Terezia; Hussein, Hussam Al; Hussein, Hamida Al; Suciu, Horatiu; Simionescu, Dan

Ionela Movileanu, Klara Brinzaniuc, Marius M. Harpa, Ovidiu S. Cotoi, Terezia Preda, Hussam Al Hussein, Hamida Al Hussein, Horatiu Suciu, Dan Simionescu

Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search

Abstract:

Aim: Our long-term aim is to develop a living valvular substitute using Regenerative Medicine principles, by seeding decellularized porcine heart valve scaffolds with adult stem cells and conditioning them in bioreactors before implantation. In this study, adult stem cells were isolated from sheep adipose tissue (ADSCs). However, we found it impractical to use cells immediately after propagation and thus, in order to extend their availability in time, a preservation method was needed. Methods: Adipose tissue was harvested from 6 sheep. ADSCs were isolated using enzymatic agents and cultured. The cells were tested for plasticity using chondrogenic, adipogenic and osteogenic differentiation kits and then cryopreserved in DMSO at -1400C. Viability was tested after a 3 week storage using Trypan Blue Staining. Results: Ovine ADSCs exhibited excellent plasticity and differentiation potential. An average of 18 million ADSCs were obtained from each ovine, exhibiting more than 88% viability after a 3-week cryopreservation period followed by thawing. Conclusions: DSMO cryopreservation represents a suitable method for ovine ADSCs for regenerative medicine. This method expands the usage of stem cells in vitro before they are differentiated into more specialized cells, offering large numbers of usable ADSCs with minimal cell loss at any desired time point.

Keywords:
regenerative medicine; heart valve; stem cells; cryopreservation

Issue: 2020, Volume 71, Issue 3

Pages: 178-184

Publication date: 2020/4/3

https://doi.org/10.37358/RC.20.3.7986

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MOVILEANU, I., BRINZANIUC, K., HARPA, M.M., COTOI, O.S., PREDA, T., HUSSEIN, H.A., HUSSEIN, H.A., SUCIU, H., SIMIONESCU, D., Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search, Rev. Chim., 71(3), 2020, 178-184.

Vancouver
Movileanu I, Brinzaniuc K, Harpa MM, Cotoi OS, Preda T, Hussein HA, Hussein HA, Suciu H, Simionescu D. Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search. Rev. Chim.[internet]. 2020 Mar;71(3):178-184. Available from: https://doi.org/10.37358/RC.20.3.7986

APA 6th edition
Movileanu, I., Brinzaniuc, K., Harpa, M.M., Cotoi, O.S., Preda, T., Hussein, H.A.,... Simionescu, D. (2020). Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search. Revista de Chimie, 71(3), 178-184. https://doi.org/10.37358/RC.20.3.7986

Harvard
Movileanu, I., Brinzaniuc, K., Harpa, M.M., Cotoi, O.S., Preda, T., Hussein, H.A., Hussein, H.A., Suciu, H., Simionescu, D. (2020). 'Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search', Revista de Chimie, 71(3), pp. 178-184. https://doi.org/10.37358/RC.20.3.7986

IEEE
I. Movileanu, K. Brinzaniuc, M.M. Harpa, O.S. Cotoi, T. Preda, H.A. Hussein, H.A. Hussein, H. Suciu, D. Simionescu, "Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search". Revista de Chimie, vol. 71, no. 3, pp. 178-184, 2020. [online]. https://doi.org/10.37358/RC.20.3.7986

Text
Ionela Movileanu, Klara Brinzaniuc, Marius M. Harpa, Ovidiu S. Cotoi, Terezia Preda, Hussam Al Hussein, Hamida Al Hussein, Horatiu Suciu, Dan Simionescu,
Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search,
Revista de Chimie,
Volume 71, Issue 3,
2020,
Pages 178-184,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.3.7986.
(https://revistadechimie.ro/Articles.asp?ID=7986)
Keywords: regenerative medicine; heart valve; stem cells; cryopreservation

RIS
TY - JOUR
T1 - Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search
A1 - Movileanu, Ionela
A2 - Brinzaniuc, Klara
A3 - Harpa, Marius M.
A4 - Cotoi, Ovidiu S.
A5 - Preda, Terezia
A6 - Hussein, Hussam Al
A7 - Hussein, Hamida Al
A8 - Suciu, Horatiu
A9 - Simionescu, Dan
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 3
SP - 178
EP - 184
UR - https://doi.org/10.37358/RC.20.3.7986
KW - regenerative medicine
KW - heart valve
KW - stem cells
KW - cryopreservation
ER -

BibTex
@article{RevCh2020P178,
author = {Movileanu Ionela and Brinzaniuc Klara and Harpa Marius M. and Cotoi Ovidiu S. and Preda Terezia and Hussein Hussam Al and Hussein Hamida Al and Suciu Horatiu and Simionescu Dan},
title = {Cryopreservation Increases Availability and Usability of Ovine Adipose-tissue Derived Stem Cells for Regenerative Medicine Search},
journal = {Revista de Chimie},
volume = {71},
number = {3},
pages = {178-184},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.3.7986},
url = {https://revistadechimie.ro/Articles.asp?ID=7986}
}

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