Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays

Barbuceanu, Florica; Baraitareanu, Stelian; Barbuceanu, Stefania-Felicia; Predoi, Gabriel

Florica Barbuceanu, Stelian Baraitareanu, Stefania-Felicia Barbuceanu, Gabriel Predoi

Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays

Abstract:

This paper describes the current diagnostic methods of Chronic Wasting Disease (CWD) in cervides used between 2013 and 2017 in Romania. The active surveillance of CWD involves the targeted groups screening by using rapid diagnostic tests (e.g., antigen capture enzyme immunoassay). If the first test does not provide certain negative results, then the confirmatory methods have been used, i.e. histopathology, immunohistochemistry and Western immunoblotting. These tests did not lead to the detection of CWD prions (PrPCWD) in Romania. This may be due to the absence or insufficient quantity of PrPCWD in samples, below the threshold of confirmatory tests.

Keywords:
CWD; transmissible spongiform encephalopathy; surveillance; diagnostic

Issue: 2020, Volume 71, Issue 3

Pages: 480-486

Publication date: 2020/4/3

https://doi.org/10.37358/RC.20.3.8022

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BARBUCEANU, F., BARAITAREANU, S., BARBUCEANU, S., PREDOI, G., Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays, Rev. Chim., 71(3), 2020, 480-486.

Vancouver
Barbuceanu F, Baraitareanu S, Barbuceanu S, Predoi G. Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays. Rev. Chim.[internet]. 2020 Mar;71(3):480-486. Available from: https://doi.org/10.37358/RC.20.3.8022

APA 6th edition
Barbuceanu, F., Baraitareanu, S., Barbuceanu, S. & Predoi, G. (2020). Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays. Revista de Chimie, 71(3), 480-486. https://doi.org/10.37358/RC.20.3.8022

Harvard
Barbuceanu, F., Baraitareanu, S., Barbuceanu, S., Predoi, G. (2020). 'Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays', Revista de Chimie, 71(3), pp. 480-486. https://doi.org/10.37358/RC.20.3.8022

IEEE
F. Barbuceanu, S. Baraitareanu, S. Barbuceanu, G. Predoi, "Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays". Revista de Chimie, vol. 71, no. 3, pp. 480-486, 2020. [online]. https://doi.org/10.37358/RC.20.3.8022

Text
Florica Barbuceanu, Stelian Baraitareanu, Stefania-Felicia Barbuceanu, Gabriel Predoi,
Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays,
Revista de Chimie,
Volume 71, Issue 3,
2020,
Pages 480-486,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.3.8022.
(https://revistadechimie.ro/Articles.asp?ID=8022)
Keywords: CWD; transmissible spongiform encephalopathy; surveillance; diagnostic

RIS
TY - JOUR
T1 - Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays
A1 - Barbuceanu, Florica
A2 - Baraitareanu, Stelian
A3 - Barbuceanu, Stefania-Felicia
A4 - Predoi, Gabriel
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 3
SP - 480
EP - 486
UR - https://doi.org/10.37358/RC.20.3.8022
KW - CWD
KW - transmissible spongiform encephalopathy
KW - surveillance
KW - diagnostic
ER -

BibTex
@article{RevCh2020P480,
author = {Barbuceanu Florica and Baraitareanu Stelian and Barbuceanu Stefania-Felicia and Predoi Gabriel},
title = {Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays},
journal = {Revista de Chimie},
volume = {71},
number = {3},
pages = {480-486},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.3.8022},
url = {https://revistadechimie.ro/Articles.asp?ID=8022}
}

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