Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield

Wang, Yonggang; Dou, Shengcai; Zhang, Qingfang; Ebadi, Abdolghaffar; Chen, Jixiang; Toughani, Mohsen

Yonggang Wang, Shengcai Dou, Qingfang Zhang, Abdolghaffar Ebadi, Jixiang Chen, Mohsen Toughani

Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield

Abstract:

The problem of environmental pollution caused by the development and use of petroleum is increasingly obvious, which is a serious threat to human health. The use of microbial degradation to treat oil pollution is one of the environmentally effective, economical and practical methods.In order to explore the soil microbial diversity in the desert area of Northwest China, this paper analyzes the soil bacterial diversity of soil samples collected from different oil-contaminated areas in Yumen Oilfield for the oil pollution problem in the Yumen Oilfield in the northwest desert area, and selects the high efficiency through pure culture technology. Petroleum degradation bacteria, and research on the biological characteristics of degrading bacteria. The composition, abundance and diversity of bacterial communities in oil-contaminated soil in Yumen Oilfield were analyzed. The culturable bacteria in western oil-contaminated desert soil were separated by coating plate method. The bacterial morphology and 16S rRNA gene system development analysis were studied. The structure and diversity of bacterial community could be cultured, and the oil utilization and degradation ability of the strain could be analyzed. The microbial diversity of Yumen oil-contaminated desert soil was analyzed by Illumina Miseq high-throughput sequencing. Through research, it is found that there are abundant bacterial groups in the oil-contaminated desert soil, and there are obvious diversity. The genetic material in the variable regions of the six soil samples detected a total of 3943 0TU at 97% similarity level, and obtained the soil microbial community. Doors, 48 classes, 78 orders, 179 families and 471 genera, including most common high-efficiency petroleum-degrading bacteria. Petroleum hydrocarbon pollution can change the microbial diversity and community structure of the original soil. The size of microbial diversity in the six soil samples is B2]A1]B1]A2]C1]C2, the diversity of B2 is the highest, the diversity of C2 is the lowest, and the microbial diversity differed greatly between groups, and there was no difference in the group. Among the dominant bacteria isolated from contaminated soil, 8 strains of oil have a degradation rate of more than 30%, including the species of the genus Rhodococcus and Pseudomonas. Soil desertification in western China has a great impact on the local ecological environment. Studying the microbial diversity of desert soils and separating high-efficiency petroleum-degrading strains is of great significance for strengthening the ecological restoration of oil-contaminated environment in desert areas.

Keywords:
oil pollution; microbial diversity; 16S rRNA; high-throughput sequencing; functional expression

Issue: 2020, Volume 71, Issue 3

Pages: 595-607

Publication date: 2020/4/3

https://doi.org/10.37358/RC.20.3.8035

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
WANG, Y., DOU, S., ZHANG, Q., EBADI, A., CHEN, J., TOUGHANI, M., Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield, Rev. Chim., 71(3), 2020, 595-607.

Vancouver
Wang Y, Dou S, Zhang Q, Ebadi A, Chen J, Toughani M. Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield. Rev. Chim.[internet]. 2020 Mar;71(3):595-607. Available from: https://doi.org/10.37358/RC.20.3.8035

APA 6th edition
Wang, Y., Dou, S., Zhang, Q., Ebadi, A., Chen, J. & Toughani, M. (2020). Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield. Revista de Chimie, 71(3), 595-607. https://doi.org/10.37358/RC.20.3.8035

Harvard
Wang, Y., Dou, S., Zhang, Q., Ebadi, A., Chen, J., Toughani, M. (2020). 'Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield', Revista de Chimie, 71(3), pp. 595-607. https://doi.org/10.37358/RC.20.3.8035

IEEE
Y. Wang, S. Dou, Q. Zhang, A. Ebadi, J. Chen, M. Toughani, "Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield". Revista de Chimie, vol. 71, no. 3, pp. 595-607, 2020. [online]. https://doi.org/10.37358/RC.20.3.8035

Text
Yonggang Wang, Shengcai Dou, Qingfang Zhang, Abdolghaffar Ebadi, Jixiang Chen, Mohsen Toughani,
Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield,
Revista de Chimie,
Volume 71, Issue 3,
2020,
Pages 595-607,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.3.8035.
(https://revistadechimie.ro/Articles.asp?ID=8035)
Keywords: oil pollution; microbial diversity; 16S rRNA; high-throughput sequencing; functional expression

RIS
TY - JOUR
T1 - Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield
A1 - Wang, Yonggang
A2 - Dou, Shengcai
A3 - Zhang, Qingfang
A4 - Ebadi, Abdolghaffar
A5 - Chen, Jixiang
A6 - Toughani, Mohsen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 3
SP - 595
EP - 607
UR - https://doi.org/10.37358/RC.20.3.8035
KW - oil pollution
KW - microbial diversity
KW - 16S rRNA
KW - high-throughput sequencing
KW - functional expression
ER -

BibTex
@article{RevCh2020P595,
author = {Wang Yonggang and Dou Shengcai and Zhang Qingfang and Ebadi Abdolghaffar and Chen Jixiang and Toughani Mohsen},
title = {Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield},
journal = {Revista de Chimie},
volume = {71},
number = {3},
pages = {595-607},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.3.8035},
url = {https://revistadechimie.ro/Articles.asp?ID=8035}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)