Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma

Bratu, Tiberiu; Berceanu, Mircea; Coricovac, Dorina; Crainiceanu, Zorin; Gyori, Zsolt; Roman, Andreea; Marcovici, Iasmina; Dehelean, Cristina; Pinzaru, Iulia

Tiberiu Bratu, Mircea Berceanu, Dorina Coricovac, Zorin Crainiceanu, Zsolt Gyori, Andreea Roman, Iasmina Marcovici, Cristina Dehelean, Iulia Pinzaru

Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma

Abstract:

In the last decade, the incidence of skin cancer has increased significantly and there is data that rank it as the most common form of malignancy in humans. Among the classified skin cancers, melanoma is the most severe with a significant mortality rate. The determining factor in the occurrence of skin cancer is generally recognised to be ultraviolet radiation. Skin pigmentation has been shown to provide a high degree of protection and this is due to melanin. In order to be able to consider certain hypotheses and to develop new approaches to melanoma, in this study it was studied the melanin content in certain cell types that have a direct connection with melanoma. The cell lines used in the study were: (i) human melanoma cell lines: A375, SK-MEL-1, SK-MEL-3, SK-MEL-5, SK-MEL-28, SH-4, and COLO 829; (ii) mouse melanoma cell lines: B164A5, B16F0, and B16F10; and (iii) healthy cell lines of human origin: HEMa (primary melanocytes), HaCaT (immortalised keratinocytes) and 1BR3 (human fibroblasts). The results obtained pointed out that A375, SK-MEL-28 and SK-MEL-5 cells do not produce melanin, SK-MEL-1, SH-4 and SK-MEL-3 cells produce melanin while COLO 829 cells - only in small passages produce melanin. Of the 3 lines of murine melanoma tested, the one that produces melanin in a higher concentration is B16F10, followed by B16F0 and B164A5.

Keywords:
melanin; content; cells; skin; melanoma

Issue: 2020, Volume 71, Issue 4

Pages: 430-437

Publication date: 2020/5/5

https://doi.org/10.37358/RC.20.4.8084

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BRATU, T., BERCEANU, M., CORICOVAC, D., CRAINICEANU, Z., GYORI, Z., ROMAN, A., MARCOVICI, I., DEHELEAN, C., PINZARU, I., Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma, Rev. Chim., 71(4), 2020, 430-437.

Vancouver
Bratu T, Berceanu M, Coricovac D, Crainiceanu Z, Gyori Z, Roman A, Marcovici I, Dehelean C, Pinzaru I. Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma. Rev. Chim.[internet]. 2020 Apr;71(4):430-437. Available from: https://doi.org/10.37358/RC.20.4.8084

APA 6th edition
Bratu, T., Berceanu, M., Coricovac, D., Crainiceanu, Z., Gyori, Z., Roman, A.,... Pinzaru, I. (2020). Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma. Revista de Chimie, 71(4), 430-437. https://doi.org/10.37358/RC.20.4.8084

Harvard
Bratu, T., Berceanu, M., Coricovac, D., Crainiceanu, Z., Gyori, Z., Roman, A., Marcovici, I., Dehelean, C., Pinzaru, I. (2020). 'Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma', Revista de Chimie, 71(4), pp. 430-437. https://doi.org/10.37358/RC.20.4.8084

IEEE
T. Bratu, M. Berceanu, D. Coricovac, Z. Crainiceanu, Z. Gyori, A. Roman, I. Marcovici, C. Dehelean, I. Pinzaru, "Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma". Revista de Chimie, vol. 71, no. 4, pp. 430-437, 2020. [online]. https://doi.org/10.37358/RC.20.4.8084

Text
Tiberiu Bratu, Mircea Berceanu, Dorina Coricovac, Zorin Crainiceanu, Zsolt Gyori, Andreea Roman, Iasmina Marcovici, Cristina Dehelean, Iulia Pinzaru,
Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma,
Revista de Chimie,
Volume 71, Issue 4,
2020,
Pages 430-437,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.4.8084.
(https://revistadechimie.ro/Articles.asp?ID=8084)
Keywords: melanin; content; cells; skin; melanoma

RIS
TY - JOUR
T1 - Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma
A1 - Bratu, Tiberiu
A2 - Berceanu, Mircea
A3 - Coricovac, Dorina
A4 - Crainiceanu, Zorin
A5 - Gyori, Zsolt
A6 - Roman, Andreea
A7 - Marcovici, Iasmina
A8 - Dehelean, Cristina
A9 - Pinzaru, Iulia
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 4
SP - 430
EP - 437
UR - https://doi.org/10.37358/RC.20.4.8084
KW - melanin
KW - content
KW - cells
KW - skin
KW - melanoma
ER -

BibTex
@article{RevCh2020P430,
author = {Bratu Tiberiu and Berceanu Mircea and Coricovac Dorina and Crainiceanu Zorin and Gyori Zsolt and Roman Andreea and Marcovici Iasmina and Dehelean Cristina and Pinzaru Iulia},
title = {Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma},
journal = {Revista de Chimie},
volume = {71},
number = {4},
pages = {430-437},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.4.8084},
url = {https://revistadechimie.ro/Articles.asp?ID=8084}
}

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