Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies <br>in Pediatric Infection with Helicobacter Pylori

Iftimi, Raluca Suzana; Stanescu, Ana Maria Alexandra; Anton, Carmen; Diaconescu, Smaranda; Gimiga, Nicoleta; Rosu, Oana-Maria

Raluca Suzana Iftimi, Ana Maria Alexandra Stanescu, Carmen Anton, Smaranda Diaconescu, Nicoleta Gimiga, Oana-Maria Rosu

Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori

Abstract:

The current methods for the diagnosis of Helicobacter pylori infection in children include invasive (direct) methods, that enable the detection of the bacteria or the bacterial urease in gastric biopsies, or noninvasive (indirect) methods, which consist of researching specific antibodies, antigens and urease in other different samples (serum, saliva, urine, stool, exhaled air). The urease functions in H. pylori infection is to neutralize gastric acid by producing NH3 through the hydrolysis of urea. Monochloramine, a NH3-derived compounds has cytotoxic effects on host cells. The Western Blot method refers to the extraction of a a sodium dodecyl sulphate from a Helicobacter pylori strain followed by a separation of the solubilised protein using discontinous polyacrylamide gel electrophoresis according to molecular mass and transfer of the separated proteins to nitrocellulose. The ELISA (enzyme-linked immunosorbent assay) technique refers to the detection of Anti-Helicobacter pylori antibodies type Ig G. We also used an immunochromatographic system with the lateral leakage test strip based on the principle of the sandwich technique with monoclonal antibodies. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold -labelled antibodies and complementary oligonucleotide-labelled immobilized at different places on a nitrocellulose membrane. Our study aims to assess the performances of the Western Blot method for determining anti-Helicobacter pylori Ig A and Ig G antibodies in correlation with clinical data and other available diagnosis methods. For validation purposes, the results were compared to those obtained using the enzyme-linked immunosorbent assay technique, gastric biopsy and immunochromatography.

Keywords:
Helicobacter pylori; Western Blot; ELISA; biopsy; children

Issue: 2020, Volume 71, Issue 4

Pages: 446-455

Publication date: 2020/5/5

https://doi.org/10.37358/RC.20.4.8087

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
IFTIMI, R.S., STANESCU, A.M.A., ANTON, C., DIACONESCU, S., GIMIGA, N., ROSU, O., Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori, Rev. Chim., 71(4), 2020, 446-455.

Vancouver
Iftimi RS, Stanescu AMA, Anton C, Diaconescu S, Gimiga N, Rosu O. Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori. Rev. Chim.[internet]. 2020 Apr;71(4):446-455. Available from: https://doi.org/10.37358/RC.20.4.8087

APA 6th edition
Iftimi, R.S., Stanescu, A.M.A., Anton, C., Diaconescu, S., Gimiga, N. & Rosu, O. (2020). Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori. Revista de Chimie, 71(4), 446-455. https://doi.org/10.37358/RC.20.4.8087

Harvard
Iftimi, R.S., Stanescu, A.M.A., Anton, C., Diaconescu, S., Gimiga, N., Rosu, O. (2020). 'Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori', Revista de Chimie, 71(4), pp. 446-455. https://doi.org/10.37358/RC.20.4.8087

IEEE
R.S. Iftimi, A.M.A. Stanescu, C. Anton, S. Diaconescu, N. Gimiga, O. Rosu, "Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori". Revista de Chimie, vol. 71, no. 4, pp. 446-455, 2020. [online]. https://doi.org/10.37358/RC.20.4.8087

Text
Raluca Suzana Iftimi, Ana Maria Alexandra Stanescu, Carmen Anton, Smaranda Diaconescu, Nicoleta Gimiga, Oana-Maria Rosu,
Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori,
Revista de Chimie,
Volume 71, Issue 4,
2020,
Pages 446-455,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.4.8087.
(https://revistadechimie.ro/Articles.asp?ID=8087)
Keywords: Helicobacter pylori; Western Blot; ELISA; biopsy; children

RIS
TY - JOUR
T1 - Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori
A1 - Iftimi, Raluca Suzana
A2 - Stanescu, Ana Maria Alexandra
A3 - Anton, Carmen
A4 - Diaconescu, Smaranda
A5 - Gimiga, Nicoleta
A6 - Rosu, Oana-Maria
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 4
SP - 446
EP - 455
UR - https://doi.org/10.37358/RC.20.4.8087
KW - Helicobacter pylori
KW - Western Blot
KW - ELISA
KW - biopsy
KW - children
ER -

BibTex
@article{RevCh2020P446,
author = {Iftimi Raluca Suzana and Stanescu Ana Maria Alexandra and Anton Carmen and Diaconescu Smaranda and Gimiga Nicoleta and Rosu Oana-Maria},
title = {Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies
in Pediatric Infection with Helicobacter Pylori},
journal = {Revista de Chimie},
volume = {71},
number = {4},
pages = {446-455},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.4.8087},
url = {https://revistadechimie.ro/Articles.asp?ID=8087}
}

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