The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions

Belei, Oana; Dobrescu, Andreea; Iacob, Emil Radu; Iacob, Daniela; Amaricai, Elena; Marginean, Otilia

Oana Belei, Andreea Dobrescu, Emil Radu Iacob, Daniela Iacob, Elena Amaricai, Otilia Marginean

The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions

Abstract:

The aim was to determine the prevalence of celiac disease (CD) in a pediatric population with juvenile idiopathic arthritis (JIA) and autoimmune hepatitis (AIH) compared to controls and to evaluate the clinical forms and human leukocyte antigen (HLA) alelles. Between September 2009-November 2019, 74 pediatric patients with JIA (1), 62 AIH (2) and 60 controls were assessed for CD. All children with one or more positive CD antibodies were submitted to gastrointestinal endoscopy with intestinal biopsy. All patients underwent HLA molecular assessment for DQ2/DQ8 alleles. Celiac prevalence after screening was 6.7% in the first group, 6.4% in the second group and 0% among controls. The results didn’t reveal significant differences regarding the CD prevalence among patients with JIA and AIH (p = 0.94). The majority of cases associated the silent form of disease (77.7). DQ2/DQ8 haplotypes were found in all CD cases. Of 69 children with JIA and no CD, three (4.3%) had DQ2 haplotype. Of 58 patients with AIH and no CD, 37 children (63.8%) presented DQ2/DQ8. According to the high prevalence obtained in this study, JIA and AIH are good parameters for stratification of asymptomatic cases in order to perform CD serologic screening. The absence of DQ2 or DQ8 haplotypes will make CD diagnosis unlikely and no further tests will be required.

Keywords:
children; juvenile idiopathic arthritis; autoimmune hepatitis; celiac disease

Issue: 2020, Volume 71, Issue 6

Pages: 212-221

Publication date: 2020/7/1

https://doi.org/10.37358/RC.20.6.8185

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BELEI, O., DOBRESCU, A., IACOB, E.R., IACOB, D., AMARICAI, E., MARGINEAN, O., The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions, Rev. Chim., 71(6), 2020, 212-221.

Vancouver
Belei O, Dobrescu A, Iacob ER, Iacob D, Amaricai E, Marginean O. The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions. Rev. Chim.[internet]. 2020 Jun;71(6):212-221. Available from: https://doi.org/10.37358/RC.20.6.8185

APA 6th edition
Belei, O., Dobrescu, A., Iacob, E.R., Iacob, D., Amaricai, E. & Marginean, O. (2020). The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions. Revista de Chimie, 71(6), 212-221. https://doi.org/10.37358/RC.20.6.8185

Harvard
Belei, O., Dobrescu, A., Iacob, E.R., Iacob, D., Amaricai, E., Marginean, O. (2020). 'The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions', Revista de Chimie, 71(6), pp. 212-221. https://doi.org/10.37358/RC.20.6.8185

IEEE
O. Belei, A. Dobrescu, E.R. Iacob, D. Iacob, E. Amaricai, O. Marginean, "The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions". Revista de Chimie, vol. 71, no. 6, pp. 212-221, 2020. [online]. https://doi.org/10.37358/RC.20.6.8185

Text
Oana Belei, Andreea Dobrescu, Emil Radu Iacob, Daniela Iacob, Elena Amaricai, Otilia Marginean,
The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions,
Revista de Chimie,
Volume 71, Issue 6,
2020,
Pages 212-221,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.6.8185.
(https://revistadechimie.ro/Articles.asp?ID=8185)
Keywords: children; juvenile idiopathic arthritis; autoimmune hepatitis; celiac disease

RIS
TY - JOUR
T1 - The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions
A1 - Belei, Oana
A2 - Dobrescu, Andreea
A3 - Iacob, Emil Radu
A4 - Iacob, Daniela
A5 - Amaricai, Elena
A6 - Marginean, Otilia
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 6
SP - 212
EP - 221
UR - https://doi.org/10.37358/RC.20.6.8185
KW - children
KW - juvenile idiopathic arthritis
KW - autoimmune hepatitis
KW - celiac disease
ER -

BibTex
@article{RevCh2020P212,
author = {Belei Oana and Dobrescu Andreea and Iacob Emil Radu and Iacob Daniela and Amaricai Elena and Marginean Otilia},
title = {The Role of Molecular Typing for DQ2 and DQ8 Alleles Using Polymerase Chain Reaction Amplification in Children with Autoimmune Conditions},
journal = {Revista de Chimie},
volume = {71},
number = {6},
pages = {212-221},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.6.8185},
url = {https://revistadechimie.ro/Articles.asp?ID=8185}
}

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