Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique

Pirvu, Florinela; Iancu, Vasile Ion; Niculescu, Marcela; Lehr, Carol Blaziu; Pascu, Luoana Florentina; Galaon, Toma

Florinela Pirvu, Vasile Ion Iancu, Marcela Niculescu, Carol Blaziu Lehr, Luoana Florentina Pascu, Toma Galaon

Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique

Abstract:

A newly developed direct injection HPLC-DAD method for the simultaneous detection of Brilliant Blue (BB), Sunset Yellow (SY) and Tartrazine (TA) synthetic dyes was validated and applied to surface and wastewater samples. Chromatographic separation was achieved using an Acclaim Surfactant Plus column (150 x 3.0 mm, 3 um) in isocratic elution mode with a mobile phase consisting of 100 mM CH3COONH4 and ACN in the ratio 50/50 (v/v). Linear calibration curves were plotted in the range of 2-100 μg/L (BB) and 6-300 μg/L (SY, TA) respectively with correlation coefficients (R2) higher than 0.99. Low quantitation limit values (LOQ) were obtained for the direct injection HPLC-DAD method, namely: 1.0 µg/L (BB), 5.5 µg/L (SY) and 5.8 µg/L (TA), respectively. The entire sample preparation procedure consisted only in simple filtration using PTFE syringe filters (0.45 µm) without sample extraction or concentration, which improved method accuracy and precision. HPLC-DAD developed method proved to be fast (6 minutes run-time), sensitive and accurate and was succesfully applied for the analysis of the synthetic dyes in surface and wastewater samples. Considering all analyzed samples (8 surface water and 11 wastewater samples), Brilliant Blue was found in influent samples with values between 4.49 ÷ 14.17 µg/L and effluent samples with values between 1.07 ÷ 9.54 µg/L, whereas Sunset Yellow and Tartrazine were not detected in any of the analyzed samples.

Keywords:
Brilliant Blue; Sunset Yellow; Tartrazine; HPLC-DAD; direct injection; surface water; wastewater

Issue: 2020, Volume 71, Issue 6

Pages: 390-400

Publication date: 2020/7/1

https://doi.org/10.37358/RC.20.6.8205

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
PIRVU, F., IANCU, V.I., NICULESCU, M., LEHR, C.B., PASCU, L.F., GALAON, T., Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique, Rev. Chim., 71(6), 2020, 390-400.

Vancouver
Pirvu F, Iancu VI, Niculescu M, Lehr CB, Pascu LF, Galaon T. Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique. Rev. Chim.[internet]. 2020 Jun;71(6):390-400. Available from: https://doi.org/10.37358/RC.20.6.8205

APA 6th edition
Pirvu, F., Iancu, V.I., Niculescu, M., Lehr, C.B., Pascu, L.F. & Galaon, T. (2020). Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique. Revista de Chimie, 71(6), 390-400. https://doi.org/10.37358/RC.20.6.8205

Harvard
Pirvu, F., Iancu, V.I., Niculescu, M., Lehr, C.B., Pascu, L.F., Galaon, T. (2020). 'Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique', Revista de Chimie, 71(6), pp. 390-400. https://doi.org/10.37358/RC.20.6.8205

IEEE
F. Pirvu, V.I. Iancu, M. Niculescu, C.B. Lehr, L.F. Pascu, T. Galaon, "Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique". Revista de Chimie, vol. 71, no. 6, pp. 390-400, 2020. [online]. https://doi.org/10.37358/RC.20.6.8205

Text
Florinela Pirvu, Vasile Ion Iancu, Marcela Niculescu, Carol Blaziu Lehr, Luoana Florentina Pascu, Toma Galaon,
Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique,
Revista de Chimie,
Volume 71, Issue 6,
2020,
Pages 390-400,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.6.8205.
(https://revistadechimie.ro/Articles.asp?ID=8205)
Keywords: Brilliant Blue; Sunset Yellow; Tartrazine; HPLC-DAD; direct injection; surface water; wastewater

RIS
TY - JOUR
T1 - Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique
A1 - Pirvu, Florinela
A2 - Iancu, Vasile Ion
A3 - Niculescu, Marcela
A4 - Lehr, Carol Blaziu
A5 - Pascu, Luoana Florentina
A6 - Galaon, Toma
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 6
SP - 390
EP - 400
UR - https://doi.org/10.37358/RC.20.6.8205
KW - Brilliant Blue
KW - Sunset Yellow
KW - Tartrazine
KW - HPLC-DAD
KW - direct injection
KW - surface water
KW - wastewater
ER -

BibTex
@article{RevCh2020P390,
author = {Pirvu Florinela and Iancu Vasile Ion and Niculescu Marcela and Lehr Carol Blaziu and Pascu Luoana Florentina and Galaon Toma},
title = {Environmental Detection of Brilliant Blue, Sunset Yellow and Tartrazine Using Direct Injection HPLC-DAD Technique},
journal = {Revista de Chimie},
volume = {71},
number = {6},
pages = {390-400},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.6.8205},
url = {https://revistadechimie.ro/Articles.asp?ID=8205}
}

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