Immunohistochemical Diagnosis  Method in Parvovirus

Stancu, Adrian; Ghise, Alina; Pente, Marius; Pasca, Sorin; Carpinisan, Liliana; Petcu, Mihaela; Degi, Janos; Berceanu-Vaduva, Delia Mira; Velimirovici, Dana Emilia; Cristina, Romeo Teodor; Tulcan, Camelia

Adrian Stancu, Alina Ghise, Marius Pente, Sorin Pasca, Liliana Carpinisan, Mihaela Petcu, Janos Degi, Delia Mira Berceanu-Vaduva, Dana Emilia Velimirovici, Romeo Teodor Cristina, Camelia Tulcan

Immunohistochemical Diagnosis Method in Parvovirus

Abstract:

10 cases of histopathologically suggestive lesions for parvovirus in the canine species were examined by the avidin-biotin-peroxidase complex. For this, a mouse monoclonal antibody was used. Following the immunohistochemical examination, positive results were obtained in 80% (8 dogs) of processed samples. The most frequent positive immunohistochemical reaction was observed in macrophages, lymphocytes, necrotic cells of the intestinal crypt, evidence of parvoviral antigen is reliable for determining the etiology of the disease if parvovirus is suspected on the basis of the lesions. In order to perform the diagnostic immunohistochemical procedure, the strepto-avidin-biotin-peroxidase method was used, using the monoclonal antibody. It is of murine origin. Negative cases of parvovirus by immunohistochemical examination show the existence of other causes that produce the same lesions; coronaviruses, rotaviruses or coronaviruses associated with clostridium perfringens which may cause intestinal necrosis of the crypt. All dog breeds can do this disease, but in the Poodle breed the disease has been more common. Sex is not a criterion of receptivity, but in proportion of over 60% were affected females. Regarding the seasonal evolution, the disease is more common in the winter period compared to the other seasons.

Keywords:
immunohistochimical; diagnosis; dogs; enteric

Issue: 2020, Volume 71, Issue 7

Pages: 491-496

Publication date: 2020/8/4

https://doi.org/10.37358/RC.20.7.8268

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
STANCU, A., GHISE, A., PENTE, M., PASCA, S., CARPINISAN, L., PETCU, M., DEGI, J., BERCEANU-VADUVA, D.M., VELIMIROVICI, D.E., CRISTINA, R.T., TULCAN, C., Immunohistochemical Diagnosis Method in Parvovirus, Rev. Chim., 71(7), 2020, 491-496.

Vancouver
Stancu A, Ghise A, Pente M, Pasca S, Carpinisan L, Petcu M, Degi J, Berceanu-Vaduva DM, Velimirovici DE, Cristina RT, Tulcan C. Immunohistochemical Diagnosis Method in Parvovirus. Rev. Chim.[internet]. 2020 Jul;71(7):491-496. Available from: https://doi.org/10.37358/RC.20.7.8268

APA 6th edition
Stancu, A., Ghise, A., Pente, M., Pasca, S., Carpinisan, L., Petcu, M.,... Tulcan, C. (2020). Immunohistochemical Diagnosis Method in Parvovirus. Revista de Chimie, 71(7), 491-496. https://doi.org/10.37358/RC.20.7.8268

Harvard
Stancu, A., Ghise, A., Pente, M., Pasca, S., Carpinisan, L., Petcu, M., Degi, J., Berceanu-Vaduva, D.M., Velimirovici, D.E., Cristina, R.T., Tulcan, C. (2020). 'Immunohistochemical Diagnosis Method in Parvovirus', Revista de Chimie, 71(7), pp. 491-496. https://doi.org/10.37358/RC.20.7.8268

IEEE
A. Stancu, A. Ghise, M. Pente, S. Pasca, L. Carpinisan, M. Petcu, J. Degi, D.M. Berceanu-Vaduva, D.E. Velimirovici, R.T. Cristina, C. Tulcan, "Immunohistochemical Diagnosis Method in Parvovirus". Revista de Chimie, vol. 71, no. 7, pp. 491-496, 2020. [online]. https://doi.org/10.37358/RC.20.7.8268

Text
Adrian Stancu, Alina Ghise, Marius Pente, Sorin Pasca, Liliana Carpinisan, Mihaela Petcu, Janos Degi, Delia Mira Berceanu-Vaduva, Dana Emilia Velimirovici, Romeo Teodor Cristina, Camelia Tulcan,
Immunohistochemical Diagnosis Method in Parvovirus,
Revista de Chimie,
Volume 71, Issue 7,
2020,
Pages 491-496,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.7.8268.
(https://revistadechimie.ro/Articles.asp?ID=8268)
Keywords: immunohistochimical; diagnosis; dogs; enteric

RIS
TY - JOUR
T1 - Immunohistochemical Diagnosis Method in Parvovirus
A1 - Stancu, Adrian
A2 - Ghise, Alina
A3 - Pente, Marius
A4 - Pasca, Sorin
A5 - Carpinisan, Liliana
A6 - Petcu, Mihaela
A7 - Degi, Janos
A8 - Berceanu-Vaduva, Delia Mira
A9 - Velimirovici, Dana Emilia
A10 - Cristina, Romeo Teodor
A11 - Tulcan, Camelia
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 7
SP - 491
EP - 496
UR - https://doi.org/10.37358/RC.20.7.8268
KW - immunohistochimical
KW - diagnosis
KW - dogs
KW - enteric
ER -

BibTex
@article{RevCh2020P491,
author = {Stancu Adrian and Ghise Alina and Pente Marius and Pasca Sorin and Carpinisan Liliana and Petcu Mihaela and Degi Janos and Berceanu-Vaduva Delia Mira and Velimirovici Dana Emilia and Cristina Romeo Teodor and Tulcan Camelia},
title = {Immunohistochemical Diagnosis Method in Parvovirus},
journal = {Revista de Chimie},
volume = {71},
number = {7},
pages = {491-496},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.7.8268},
url = {https://revistadechimie.ro/Articles.asp?ID=8268}
}

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