A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

Bouzid, Habib; Filho, Cesar M. C.; Marques, Joana Rita C.; Valente, Artur J. M.; Gando-Ferreira, Licinio M.

Habib Bouzid, Cesar M. C. Filho, Joana Rita C. Marques, Artur J. M. Valente, Licinio M. Gando-Ferreira

A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry

Abstract:

The methodology of the amino acid lysine (Lys) analysis using ninhydrin (Nin) reagent by spectrophotometric method was in-depth studied and improved. The optimization of time, Lys to Nin volume ratio and temperature were explicitly investigated. A specific wavelength of 479 nm was set according to the peak of the absorbance spectrum. The reaction time between Lys and Nin, in aqueous solution, occurs during 50 min. The Lys/Nin ratio 1.67 appeared most effective for this reaction. Moreover, the absorbance increased with temperature until a stable value was achieved nearly at 85°C. It was also found that the reaction is more efficient at initial pH 6 corresponding to formation of high amount of coloured product. Furthermore, the pH of post-reaction mixture was around 4 for different concentrations of lysine and initial pH range 3-10. For lysine solution contaminated with potassium (a representative interfering species) and after variation of the reaction conditions, the maximum wavelength (479 nm) was not affected. Mastering the use of these parameters leads to a good usage of this analytical technique which is simple, fast, accurate, less expensive and especially less harmful towards the environment and human health.

Keywords:
amino acid lysine; ninhydrin reagent; UV-Visible spectrophotometric analysis; optimization

Issue: 2020, Volume 71, Issue 8

Pages: 159-175

Publication date: 2020/8/31

https://doi.org/10.37358/RC.20.8.8290

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BOUZID, H., FILHO, C.M.C., MARQUES, J.R.C., VALENTE, A.J.M., GANDO-FERREIRA, L.M., A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry, Rev. Chim., 71(8), 2020, 159-175.

Vancouver
Bouzid H, Filho CMC, Marques JRC, Valente AJM, Gando-Ferreira LM. A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry. Rev. Chim.[internet]. 2020 Aug;71(8):159-175. Available from: https://doi.org/10.37358/RC.20.8.8290

APA 6th edition
Bouzid, H., Filho, C.M.C., Marques, J.R.C., Valente, A.J.M. & Gando-Ferreira, L.M. (2020). A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry. Revista de Chimie, 71(8), 159-175. https://doi.org/10.37358/RC.20.8.8290

Harvard
Bouzid, H., Filho, C.M.C., Marques, J.R.C., Valente, A.J.M., Gando-Ferreira, L.M. (2020). 'A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry', Revista de Chimie, 71(8), pp. 159-175. https://doi.org/10.37358/RC.20.8.8290

IEEE
H. Bouzid, C.M.C. Filho, J.R.C. Marques, A.J.M. Valente, L.M. Gando-Ferreira, "A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry". Revista de Chimie, vol. 71, no. 8, pp. 159-175, 2020. [online]. https://doi.org/10.37358/RC.20.8.8290

Text
Habib Bouzid, Cesar M. C. Filho, Joana Rita C. Marques, Artur J. M. Valente, Licinio M. Gando-Ferreira,
A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry,
Revista de Chimie,
Volume 71, Issue 8,
2020,
Pages 159-175,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.8.8290.
(https://revistadechimie.ro/Articles.asp?ID=8290)
Keywords: amino acid lysine; ninhydrin reagent; UV-Visible spectrophotometric analysis; optimization

RIS
TY - JOUR
T1 - A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry
A1 - Bouzid, Habib
A2 - Filho, Cesar M. C.
A3 - Marques, Joana Rita C.
A4 - Valente, Artur J. M.
A5 - Gando-Ferreira, Licinio M.
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 8
SP - 159
EP - 175
UR - https://doi.org/10.37358/RC.20.8.8290
KW - amino acid lysine
KW - ninhydrin reagent
KW - UV-Visible spectrophotometric analysis
KW - optimization
ER -

BibTex
@article{RevCh2020P159,
author = {Bouzid Habib and Filho Cesar M. C. and Marques Joana Rita C. and Valente Artur J. M. and Gando-Ferreira Licinio M.},
title = {A New Approach on the Amino Acid Lysine Quantification by UV-Visible Spectrophotometry},
journal = {Revista de Chimie},
volume = {71},
number = {8},
pages = {159-175},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.8.8290},
url = {https://revistadechimie.ro/Articles.asp?ID=8290}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)