Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells

Yu-Yuan, Li; Yi-Jun, Wu

Li Yu-Yuan, Wu Yi-Jun

Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells

Abstract:

Neuropathy target esterase (NTE), is a membrane protein located in the endoplasmic reticulum (ER). NTE has the activity of phospholipase B and can catalyze the deacylation of phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) to glycerylcholine (GPC). It is phosphorylated and aged by organophosphorus compounds (OPs), that induce delayed neuropathy in humans and sensitive animals. Our previous study has reported that the disruption of ER phospholipid homeostasis caused by the NTE inhibition may contribute to the initiation of the organophosphate-induced delayed neurotoxicity (OPIDN), while it is unknown how the disturbed phospholipid homeostasis initiates OPIDN. It is difficult to change phospholipids in in vivo experiments. Therefore, an in vitro model is urgently needed to explain the role of phospholipid homeostasis disorders in OPIDN. In this study, we altered the expression of NTE in SK-N-SH cells and determined its phospholipid component by using HPLC-MS. Our results showed that the changes of NTE affected the levels of PC, sphingomyelin (SM), phosphatidylethanolamine (PE), phosphatidylserine (PS), lysophosphatidylserine (LPS), phosphatidyl-glycerol (PG), and phosphatidylinositol (PI). Our results were consistent with the in vivo results. Furthermore, our findings indicate that the SK-N-SH cell model is a significantly useful method for the further research on how the changes of phospholipid homeostasis initiate the OPIDN, which is easier than the in vivo experiments in practice.

Keywords:
NTE; Phospholipid homeostasis; SK-N-SH cells

Issue: 2020, Volume 71, Issue 8

Pages: 327-334

Publication date: 2020/8/31

https://doi.org/10.37358/RC.20.8.8306

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
YU-YUAN, L., YI-JUN, W., Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells, Rev. Chim., 71(8), 2020, 327-334.

Vancouver
Yu-Yuan L, Yi-Jun W. Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells. Rev. Chim.[internet]. 2020 Aug;71(8):327-334. Available from: https://doi.org/10.37358/RC.20.8.8306

APA 6th edition
Yu-Yuan, L., Yi-Jun, W. (2020). Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells. Revista de Chimie, 71(8), 327-334. https://doi.org/10.37358/RC.20.8.8306

Harvard
Yu-Yuan, L., Yi-Jun, W. (2020). 'Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells', Revista de Chimie, 71(8), pp. 327-334. https://doi.org/10.37358/RC.20.8.8306

IEEE
L. Yu-Yuan, W. Yi-Jun, "Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells". Revista de Chimie, vol. 71, no. 8, pp. 327-334, 2020. [online]. https://doi.org/10.37358/RC.20.8.8306

Text
Li Yu-Yuan, Wu Yi-Jun,
Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells,
Revista de Chimie,
Volume 71, Issue 8,
2020,
Pages 327-334,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.8.8306.
(https://revistadechimie.ro/Articles.asp?ID=8306)
Keywords: NTE; Phospholipid homeostasis; SK-N-SH cells

RIS
TY - JOUR
T1 - Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells
A1 - Yu-Yuan, Li
A2 - Yi-Jun, Wu
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 8
SP - 327
EP - 334
UR - https://doi.org/10.37358/RC.20.8.8306
KW - NTE
KW - Phospholipid homeostasis
KW - SK-N-SH cells
ER -

BibTex
@article{RevCh2020P327,
author = {Yu-Yuan Li and Yi-Jun Wu},
title = {Changes of Neuropathy Target Esterase Affect Phospholipid Homeostasis in SK-N-SH Cells},
journal = {Revista de Chimie},
volume = {71},
number = {8},
pages = {327-334},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.8.8306},
url = {https://revistadechimie.ro/Articles.asp?ID=8306}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)