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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2020, Volume 71, Issue 9, 32-38

https://doi.org/10.37358/RC.20.9.8314

Kinza Nisar, Roheena Abdullah, Afshan Kaleem, Mehwish Iqtedar, Faiza Saleem, Tehreema Iftikhar, Farheen Aslam

Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii

Abstract:

A consecutive optimization based on statistical approach was applied for a-glucosidase production by both wild and mutant T. dupontii. Plackett Burman design (PBD) with two levels was employed in order to screen the significant effect of different nutritional and physical parameters through submerged fermentation. Among all nine variables tested in PBD, incubation time, inoculum size and ammonium sulphate concentration were selected. The Box-Behnken approach was further applied for process optimization. The a-glucosidase production for both wild and mutant T.dupontii was obtained at 72 h of incubation, 1.25 mL inoculum size and 0.25% ammonium sulphate concentration with relatively 95% correlation between the experimentally predicted and observed values. The duration of maximum enzyme production in RSM was cost-saving and fast. The quadratic model was in satisfactory adjustment with the experimental data with high R2 value which describes 98.90% of response variability of the model. Moreover, the novel approach of this present work is that, consecutive optimization were applied for maximum a-glucosidase production using response surface methodology by both wild and mutant thermophillic T. dupontii. Results revealed that thermophillic mutant T. dupontii could be potential candidate for industrial applications.
Keywords:
thermophillic; quadratic model; submerged fermentation; Response surface methodology; T. dupontii; a-glucosidase

Issue: 2020, Volume 71, Issue 9
Pages: 32-38
Publication date: 2020/9/5
https://doi.org/10.37358/RC.20.9.8314
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
NISAR, K., ABDULLAH, R., KALEEM, A., IQTEDAR, M., SALEEM, F., IFTIKHAR, T., ASLAM, F., Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii, Rev. Chim., 71(9), 2020, 32-38.

Vancouver
Nisar K, Abdullah R, Kaleem A, Iqtedar M, Saleem F, Iftikhar T, Aslam F. Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii. Rev. Chim.[internet]. 2020 Sep;71(9):32-38. Available from: https://doi.org/10.37358/RC.20.9.8314


APA 6th edition
Nisar, K., Abdullah, R., Kaleem, A., Iqtedar, M., Saleem, F., Iftikhar, T. & Aslam, F. (2020). Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii. Revista de Chimie, 71(9), 32-38. https://doi.org/10.37358/RC.20.9.8314


Harvard
Nisar, K., Abdullah, R., Kaleem, A., Iqtedar, M., Saleem, F., Iftikhar, T., Aslam, F. (2020). 'Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii', Revista de Chimie, 71(9), pp. 32-38. https://doi.org/10.37358/RC.20.9.8314


IEEE
K. Nisar, R. Abdullah, A. Kaleem, M. Iqtedar, F. Saleem, T. Iftikhar, F. Aslam, "Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii". Revista de Chimie, vol. 71, no. 9, pp. 32-38, 2020. [online]. https://doi.org/10.37358/RC.20.9.8314


Text
Kinza Nisar, Roheena Abdullah, Afshan Kaleem, Mehwish Iqtedar, Faiza Saleem, Tehreema Iftikhar, Farheen Aslam,
Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii,
Revista de Chimie,
Volume 71, Issue 9,
2020,
Pages 32-38,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.9.8314.
(https://revistadechimie.ro/Articles.asp?ID=8314)
Keywords: thermophillic; quadratic model; submerged fermentation; Response surface methodology; T. dupontii; a-glucosidase


RIS
TY - JOUR
T1 - Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii
A1 - Nisar, Kinza
A2 - Abdullah, Roheena
A3 - Kaleem, Afshan
A4 - Iqtedar, Mehwish
A5 - Saleem, Faiza
A6 - Iftikhar, Tehreema
A7 - Aslam, Farheen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 9
SP - 32
EP - 38
UR - https://doi.org/10.37358/RC.20.9.8314
KW - thermophillic
KW - quadratic model
KW - submerged fermentation
KW - Response surface methodology
KW - T. dupontii
KW - a-glucosidase
ER -


BibTex
@article{RevCh2020P32,
author = {Nisar Kinza and Abdullah Roheena and Kaleem Afshan and Iqtedar Mehwish and Saleem Faiza and Iftikhar Tehreema and Aslam Farheen},
title = {Application of Plackett Burman and Box Behnken Design for the Optimization of a-glucosidase Production by Thermophillic Thermomyces dupontii},
journal = {Revista de Chimie},
volume = {71},
number = {9},
pages = {32-38},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.9.8314},
url = {https://revistadechimie.ro/Articles.asp?ID=8314}
}
 
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