Studies on the Characterization of Esterase from Different  Pisum sativum L. (Fabaceae) Varieties

Murthy, Anushree Srinivasa; Manjunath, Ramya; Prakash, Tulasi Dhondale; Reddy, Mahesh Krishna; Jagarlamudi, Krishna Rao; Sathyamurthy, Balasubramanian; Tataringa, Gabriela

Anushree Srinivasa Murthy, Ramya Manjunath, Tulasi Dhondale Prakash, Mahesh Krishna Reddy, Krishna Rao Jagarlamudi, Balasubramanian Sathyamurthy, Gabriela Tataringa

Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties

Abstract:

Pisum sativum L. (Fabaceae) is important in diet due to its fiber content, protein, starch, trace elements and phytochemical substances. Dichlorvos (2, 3-dichlorovinyl dimethyl phosphate) is commonly known as organophosphates belong to the classes of insecticides that are used to control households and stored products insects. The aim of this study was to analyze the kinetic characteristics of esterase enzyme from four different varieties of Pisum sativum L. using qualitative and quantitative method and also the docking position of esterase with Dichlorvos by insilico method. According to our study the total activity was highest for Arka Nirmala and Arka Priya varieties. The specific activity was highest for Arka Mayur variety. Km and Vmax, were found to be higher for Arka Uttam. The optimum temperature and pH for all the varieties were found to be same, 40°C and 7.5 respectively. The inhibitor studies showed Arka Nirmala was more sensitive to inhibitor. From the fitness and interaction profile it was found that the inhibitor dichlorovos is an effective pesticide which can be used to inhibit esterase activity. It has one hydrogen bond of H-S and had an amino acid residue at ARG57.

Keywords:
Pisum sativum L.; esterase; dichlorvos; molecular docking

Issue: 2020, Volume 71, Issue 9

Pages: 47-54

Publication date: 2020/9/5

https://doi.org/10.37358/RC.20.9.8316

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MURTHY, A.S., MANJUNATH, R., PRAKASH, T.D., REDDY, M.K., JAGARLAMUDI, K.R., SATHYAMURTHY, B., TATARINGA, G., Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties, Rev. Chim., 71(9), 2020, 47-54.

Vancouver
Murthy AS, Manjunath R, Prakash TD, Reddy MK, Jagarlamudi KR, Sathyamurthy B, Tataringa G. Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties. Rev. Chim.[internet]. 2020 Sep;71(9):47-54. Available from: https://doi.org/10.37358/RC.20.9.8316

APA 6th edition
Murthy, A.S., Manjunath, R., Prakash, T.D., Reddy, M.K., Jagarlamudi, K.R., Sathyamurthy, B. & Tataringa, G. (2020). Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties. Revista de Chimie, 71(9), 47-54. https://doi.org/10.37358/RC.20.9.8316

Harvard
Murthy, A.S., Manjunath, R., Prakash, T.D., Reddy, M.K., Jagarlamudi, K.R., Sathyamurthy, B., Tataringa, G. (2020). 'Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties', Revista de Chimie, 71(9), pp. 47-54. https://doi.org/10.37358/RC.20.9.8316

IEEE
A.S. Murthy, R. Manjunath, T.D. Prakash, M.K. Reddy, K.R. Jagarlamudi, B. Sathyamurthy, G. Tataringa, "Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties". Revista de Chimie, vol. 71, no. 9, pp. 47-54, 2020. [online]. https://doi.org/10.37358/RC.20.9.8316

Text
Anushree Srinivasa Murthy, Ramya Manjunath, Tulasi Dhondale Prakash, Mahesh Krishna Reddy, Krishna Rao Jagarlamudi, Balasubramanian Sathyamurthy, Gabriela Tataringa,
Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties,
Revista de Chimie,
Volume 71, Issue 9,
2020,
Pages 47-54,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.9.8316.
(https://revistadechimie.ro/Articles.asp?ID=8316)
Keywords: Pisum sativum L.; esterase; dichlorvos; molecular docking

RIS
TY - JOUR
T1 - Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties
A1 - Murthy, Anushree Srinivasa
A2 - Manjunath, Ramya
A3 - Prakash, Tulasi Dhondale
A4 - Reddy, Mahesh Krishna
A5 - Jagarlamudi, Krishna Rao
A6 - Sathyamurthy, Balasubramanian
A7 - Tataringa, Gabriela
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 9
SP - 47
EP - 54
UR - https://doi.org/10.37358/RC.20.9.8316
KW - Pisum sativum L.
KW - esterase
KW - dichlorvos
KW - molecular docking
ER -

BibTex
@article{RevCh2020P47,
author = {Murthy Anushree Srinivasa and Manjunath Ramya and Prakash Tulasi Dhondale and Reddy Mahesh Krishna and Jagarlamudi Krishna Rao and Sathyamurthy Balasubramanian and Tataringa Gabriela},
title = {Studies on the Characterization of Esterase from Different Pisum sativum L. (Fabaceae) Varieties},
journal = {Revista de Chimie},
volume = {71},
number = {9},
pages = {47-54},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.9.8316},
url = {https://revistadechimie.ro/Articles.asp?ID=8316}
}

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