ABOUT INDEXING BOARD ARCHIVES AUTHOR GUIDELINES SUBMIT PAPER AUTHOR'S PAGE NEWS, EVENTS CONTACT
REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

OSIM Nr. R102355
ISSN Print 0034-7752
ISSN Online 2668-8212
Journal Metrics
* Impact Factor 2019: 1.755
* 5-Year IF: 1.351
* CiteScore: 2.2
* H index: 28
(InCites Journal Citation Reports)

REVISTA DE CHIMIE Latest Issue

LATEST ISSUE >>>

   Volume 71, 2020
   Volume 70, 2019
   Volume 69, 2018
   Volume 68, 2017
   Volume 67, 2016
   Volume 66, 2015
   Volume 65, 2014
   Volume 64, 2013
   Volume 63, 2012
   Volume 62, 2011
   Volume 61, 2010
   Volume 60, 2009
   Volume 59, 2008
   Volume 58, 2007
 
<<<< back

Revista de Chimie (Rev. Chim.), Year 2020, Volume 71, Issue 9, 230-241

https://doi.org/10.37358/RC.20.9.8333

Volodymyr Antonyuk, Lidiya Panchak, Nazar Manko, Rostislav Stoika

Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity

Abstract:

Chitosan is widely used in biology and medicine. Usually, it is isolated from chitin of crustaceans, while chitosan of the basidiomycetes fungi is poorly studied. The purpose of this study was: 1) to develop a method for isolation of chitosan from dried pomace of the peppery milky cap (Lactarius pergamenus); 2) to study of physico-chemical and biological properties of the isolated chitosan. As tradicionally, chitosan was obtained by the alkaline hydrolysis of chitin. The determination of its molecular mass was performed using the viscometric method and further analyzed by disk electrophoresis (pH 5.0). The anti-microbial and cytotoxic activities were measured spectrophoto¬metrically as a convertion of MTT dye to formazan, while the antifungal activity was evaluated using by counting colony-forming units (CFU). Chitosan of L. pergamenus fungi was shown to have lower molecular mass and a lower degree of deacetylation compared to shrimp chitosan. A yield of chitosan from a pomace of L. pergamenus was 6.27%. A heterogeneous product was obtained with an average molecular mass of 72 kDa and a degree of deacetylation equal - 87.1%. In 10 mg/ml dose, it inhibited by 29% the growth of gram-positive of Staphylococcus aureus (ATCC25923) bacteria and inhibited growth of gram-negative Echerichia coli dH5a - by 86% and Pseudomonas aeruginosa (ATCC9027) bacteria by 55%. Approximately 50 and 90 % of Candida albicans (pat.) cells were killed at the action of chitosan in doses of 0.025 mg/mL and 0.1 mg/mL correspondingly. It should be noted that this chitosan preparation did not affect a growth of human embryonic kidney pseudonormal cells of HEK 293 line and human breast carcinoma cells of MCF7 line.
Keywords:
Lactarius pergamenus; chitin; chitosan; purification; physico-chemical properties; antimicrobial activity

Issue: 2020, Volume 71, Issue 9
Pages: 230-241
Publication date: 2020/9/5
https://doi.org/10.37358/RC.20.9.8333
download pdf   Download Pdf Article
Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
ANTONYUK, V., PANCHAK, L., MANKO, N., STOIKA, R., Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity, Rev. Chim., 71(9), 2020, 230-241.

Vancouver
Antonyuk V, Panchak L, Manko N, Stoika R. Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity. Rev. Chim.[internet]. 2020 Sep;71(9):230-241. Available from: https://doi.org/10.37358/RC.20.9.8333


APA 6th edition
Antonyuk, V., Panchak, L., Manko, N. & Stoika, R. (2020). Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity. Revista de Chimie, 71(9), 230-241. https://doi.org/10.37358/RC.20.9.8333


Harvard
Antonyuk, V., Panchak, L., Manko, N., Stoika, R. (2020). 'Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity', Revista de Chimie, 71(9), pp. 230-241. https://doi.org/10.37358/RC.20.9.8333


IEEE
V. Antonyuk, L. Panchak, N. Manko, R. Stoika, "Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity". Revista de Chimie, vol. 71, no. 9, pp. 230-241, 2020. [online]. https://doi.org/10.37358/RC.20.9.8333


Text
Volodymyr Antonyuk, Lidiya Panchak, Nazar Manko, Rostislav Stoika,
Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity,
Revista de Chimie,
Volume 71, Issue 9,
2020,
Pages 230-241,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.9.8333.
(https://revistadechimie.ro/Articles.asp?ID=8333)
Keywords: Lactarius pergamenus; chitin; chitosan; purification; physico-chemical properties; antimicrobial activity


RIS
TY - JOUR
T1 - Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity
A1 - Antonyuk, Volodymyr
A2 - Panchak, Lidiya
A3 - Manko, Nazar
A4 - Stoika, Rostislav
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 9
SP - 230
EP - 241
UR - https://doi.org/10.37358/RC.20.9.8333
KW - Lactarius pergamenus
KW - chitin
KW - chitosan
KW - purification
KW - physico-chemical properties
KW - antimicrobial activity
ER -


BibTex
@article{RevCh2020P230,
author = {Antonyuk Volodymyr and Panchak Lidiya and Manko Nazar and Stoika Rostislav},
title = {Chitosan of Peppery Milky Cap Fungi (Lactarius Pergamenus (Fr.) Fr): Isolation, Study of Physico-Chemical Properties and Biological Activity},
journal = {Revista de Chimie},
volume = {71},
number = {9},
pages = {230-241},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.9.8333},
url = {https://revistadechimie.ro/Articles.asp?ID=8333}
}
 
<<<< back
 
PUBLISHING SECTION
Author Guidelines
 * Paper Drafting
 * Paper Template
 * Ethical Statement
 * Paper Submission
 * Author Payments
Ghidul Autorului (ro)
Submit Paper
Author's Page
Publishing Ethics
Peer Review Process
Open Access & Archiving Policy
 Search Authors
 LATEST NEWS
REvista de Chimie Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
dictionar English-Romanian Dictionary for Mechanical Engineering
(download pdf)
  News, Events
Crossref Member Badge
 DOI  logo
 Gold Open Access | Source=http://www.plos.org/  | Author=art designer at PLoS
Creative Commons License
 Conferences of Contemporary
 Chemistry
 Biblioteca Chimiei
 Materiale Plastice