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REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2020, Volume 71, Issue 9, 277-284

https://doi.org/10.37358/RC.20.9.8338

Adriana Gherbon, Mirela Frandes, Lavinia Noveanu, Marioara Nicula, Dorel Dronca, Mirela Ahmadi, Mihai Ionita, Romulus Timar

LC-MS/MS Method in Nicotine Metabolites Quantification

Abstract:

Nicotine is a ganglioplegic alkaloid, being the main component of tobacco. Some of the final metabolites of nicotine could be used to evaluate the nicotine dependency in humans using the Fagerstrom test. The test consists in cotinine quantitative determination, and evaluation of the correlation between cotinine from plasma and from urine. Cotinine quantification was performed using LC-MS/MS method both for blood and urine, in a private authorized laboratory for medical tests. Our study was conducted in two stages (1st year of study - initial assessment; and 6th year of study - final assessment) and included 62 apparently healthy subjects (77% females and 23% males) with an average age of 19±1.48 years at baseline, respectively 24±0.83 years at the final assessment. The prevalence of smoking increased significantly from 14.51% to 32.25% (p=0.01) (11% F and 3% M initially, respectively 25% F and 7% M final). The final assessment found that 21% became smokers after starting their university studies, 11% were permanent smokers throughout the 6th year study, and only 3.22% quit smoking. Percentage distribution on smoking addiction levels was at baseline: mild 11%, moderate 33%, severe 56%, and a final assessment: mild 15% (p=0.77), moderate 75% (p=0.01) and severe 10% (p=0.007). The results show a significant increase in the number of smoker girls compared to boys, a significant increase in the prevalence of moderately dependent smokers and a significant decrease in the prevalence of severely dependent smokers. LC-MS/MS can be used very well to evaluate the cotinine content in blood and urine, which could be used in Fagerstrom test in order to evaluate the nicotine addiction in young people and to find priority solutions for smoking prevention and quit smoking.
Keywords:
nicotine; metabolism; smokers

Issue: 2020, Volume 71, Issue 9
Pages: 277-284
Publication date: 2020/9/5
https://doi.org/10.37358/RC.20.9.8338
download pdf   Download Pdf Article
Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
GHERBON, A., FRANDES, M., NOVEANU, L., NICULA, M., DRONCA, D., AHMADI, M., IONITA, M., TIMAR, R., LC-MS/MS Method in Nicotine Metabolites Quantification, Rev. Chim., 71(9), 2020, 277-284.

Vancouver
Gherbon A, Frandes M, Noveanu L, Nicula M, Dronca D, Ahmadi M, Ionita M, Timar R. LC-MS/MS Method in Nicotine Metabolites Quantification. Rev. Chim.[internet]. 2020 Sep;71(9):277-284. Available from: https://doi.org/10.37358/RC.20.9.8338


APA 6th edition
Gherbon, A., Frandes, M., Noveanu, L., Nicula, M., Dronca, D., Ahmadi, M., Ionita, M. & Timar, R. (2020). LC-MS/MS Method in Nicotine Metabolites Quantification. Revista de Chimie, 71(9), 277-284. https://doi.org/10.37358/RC.20.9.8338


Harvard
Gherbon, A., Frandes, M., Noveanu, L., Nicula, M., Dronca, D., Ahmadi, M., Ionita, M., Timar, R. (2020). 'LC-MS/MS Method in Nicotine Metabolites Quantification', Revista de Chimie, 71(9), pp. 277-284. https://doi.org/10.37358/RC.20.9.8338


IEEE
A. Gherbon, M. Frandes, L. Noveanu, M. Nicula, D. Dronca, M. Ahmadi, M. Ionita, R. Timar, "LC-MS/MS Method in Nicotine Metabolites Quantification". Revista de Chimie, vol. 71, no. 9, pp. 277-284, 2020. [online]. https://doi.org/10.37358/RC.20.9.8338


Text
Adriana Gherbon, Mirela Frandes, Lavinia Noveanu, Marioara Nicula, Dorel Dronca, Mirela Ahmadi, Mihai Ionita, Romulus Timar,
LC-MS/MS Method in Nicotine Metabolites Quantification,
Revista de Chimie,
Volume 71, Issue 9,
2020,
Pages 277-284,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.9.8338.
(https://revistadechimie.ro/Articles.asp?ID=8338)
Keywords: nicotine; metabolism; smokers


RIS
TY - JOUR
T1 - LC-MS/MS Method in Nicotine Metabolites Quantification
A1 - Gherbon, Adriana
A2 - Frandes, Mirela
A3 - Noveanu, Lavinia
A4 - Nicula, Marioara
A5 - Dronca, Dorel
A6 - Ahmadi, Mirela
A7 - Ionita, Mihai
A8 - Timar, Romulus
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 9
SP - 277
EP - 284
UR - https://doi.org/10.37358/RC.20.9.8338
KW - nicotine
KW - metabolism
KW - smokers
ER -


BibTex
@article{RevCh2020P277,
author = {Gherbon Adriana and Frandes Mirela and Noveanu Lavinia and Nicula Marioara and Dronca Dorel and Ahmadi Mirela and Ionita Mihai and Timar Romulus},
title = {LC-MS/MS Method in Nicotine Metabolites Quantification},
journal = {Revista de Chimie},
volume = {71},
number = {9},
pages = {277-284},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.9.8338},
url = {https://revistadechimie.ro/Articles.asp?ID=8338}
}
 
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