Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.

Cristea, Tina Oana; Iosob, Gabriel-Alin; Brezeanu, Creola; Brezeanu, Petre Marian

Tina Oana Cristea, Gabriel-Alin Iosob, Creola Brezeanu, Petre Marian Brezeanu

Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.

Abstract:

The dimension of the bud is a key factor for the orientation of microspore culture and the success of obtaining double haploid plants as it is a strong correlation between bud size and the developmental stages of microspores, and it is specific for each plant species and genotype. Our study was focused to determine the correlation between morphological characteristics, namely floral bud size and specific microspore developmental stages in order to determine the proper size, suitable for a successful protocol of obtaining double haploid plants in Brassica oleracea var. italica. Thus, we tested four bud sizes ranging from 2.0 to 4.0 mm measured from the base to the tip of the bud. After the statistical analysis of the results it can be emphasized that the best results were obtained in the case of using as a source of microspores the flower buds with the size between 3.1-3.5 mm. At this dimension, the share of microspores in the uninucleate stage, predominantly in the late uninucleate stage, is 90%, thus ensuring a homogeneous population of microspores in the optimum stage of development. Their evolution is predominantly embryogenic, the percentage of microspores following the gametophytic pathway is reduced, by only 9.12%.

Keywords:
haploid; embryogenesis; floral; bud; vitro

Issue: 2020, Volume 71, Issue 10

Pages: 131-136

Publication date: 2020/11/3

https://doi.org/10.37358/RC.20.10.8357

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
CRISTEA, T.O., IOSOB, G., BREZEANU, C., BREZEANU, P.M., Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L., Rev. Chim., 71(10), 2020, 131-136.

Vancouver
Cristea TO, Iosob G, Brezeanu C, Brezeanu PM. Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.. Rev. Chim.[internet]. 2020 Oct;71(10):131-136. Available from: https://doi.org/10.37358/RC.20.10.8357

APA 6th edition
Cristea, T.O., Iosob, G., Brezeanu, C. & Brezeanu, P.M. (2020). Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.. Revista de Chimie, 71(10), 131-136. https://doi.org/10.37358/RC.20.10.8357

Harvard
Cristea, T.O., Iosob, G., Brezeanu, C., Brezeanu, P.M. (2020). 'Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.', Revista de Chimie, 71(10), pp. 131-136. https://doi.org/10.37358/RC.20.10.8357

IEEE
T.O. Cristea, G. Iosob, C. Brezeanu, P.M. Brezeanu, "Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.". Revista de Chimie, vol. 71, no. 10, pp. 131-136, 2020. [online]. https://doi.org/10.37358/RC.20.10.8357

Text
Tina Oana Cristea, Gabriel-Alin Iosob, Creola Brezeanu, Petre Marian Brezeanu,
Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.,
Revista de Chimie,
Volume 71, Issue 10,
2020,
Pages 131-136,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.10.8357.
(https://revistadechimie.ro/Articles.asp?ID=8357)
Keywords: haploid; embryogenesis; floral; bud; vitro

RIS
TY - JOUR
T1 - Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.
A1 - Cristea, Tina Oana
A2 - Iosob, Gabriel-Alin
A3 - Brezeanu, Creola
A4 - Brezeanu, Petre Marian
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 10
SP - 131
EP - 136
UR - https://doi.org/10.37358/RC.20.10.8357
KW - haploid
KW - embryogenesis
KW - floral
KW - bud
KW - vitro
ER -

BibTex
@article{RevCh2020P131,
author = {Cristea Tina Oana and Iosob Gabriel-Alin and Brezeanu Creola and Brezeanu Petre Marian},
title = {Effect of Chemical Composition of Nutritive Medium and Explant Size Over Androgenetic Response in Microspore Culture of Brassica oleracea L.},
journal = {Revista de Chimie},
volume = {71},
number = {10},
pages = {131-136},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.10.8357},
url = {https://revistadechimie.ro/Articles.asp?ID=8357}
}

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