Caseinase Production and Media Optimization from Bacillus subtilis

Hussein, Noor Nihad Abdul; Ibrahim, Aseel I.; Kamar, Firas Hashim; Nechifor, Arelia Cristina

Noor Nihad Abdul Hussein, Aseel I. Ibrahim, Firas Hashim Kamar, Arelia Cristina Nechifor

Caseinase Production and Media Optimization from Bacillus subtilis

Abstract:

Caseinase is involved in the breakdown of milk protein casein and converts casein into smaller simple sugars which can be easily utilized by the body for the production of ATP and Fat. Casein can be an instant energy source to the body and involves in muscle building. Caseinase enzyme can be extensively used at the industrial scale for Milk, Textile, Dairy, Paper industry and several other medical purposes. In view of the importance of caseinase, the current research deals with the isolation and identification of caseinase producing bacteria from soil. This is followed by the production of enzyme and its purification. The study also includes its kinetic characterization using the parameters Temperature, pH as well as Carbon and Nitrogen Sources. The organism which was isolated from soil and capable of producing the caseinase enzyme was identified to be Bacillus subtilis based on the Biochemical tests and 16S rRNA sequencing result. The optimal carbon and nitrogen sources were identified to be Glucose and casein respectively. Regarding the optimal conditions, the suitable temperature for maximum enzyme production was found to be 40 0C and pH was 9. When the organism was cultured under the optimal condition using casein as a nitrogen source and glucose as the carbon source, at 40 0C and pH 9, 1590 ng/mL of enzyme production was estimated.

Keywords:
Caseinase; Bacillus subtilis; kinetic characterization; 16S rRNA sequencing

Issue: 2020, Volume 71, Issue 11

Pages: 1-9

Publication date: 2020/12/4

https://doi.org/10.37358/RC.20.11.8368

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
HUSSEIN, N.N.A., IBRAHIM, A.I., KAMAR, F.H., NECHIFOR, A.C., Caseinase Production and Media Optimization from Bacillus subtilis, Rev. Chim., 71(11), 2020, 1-9.

Vancouver
Hussein NNA, Ibrahim AI, Kamar FH, Nechifor AC. Caseinase Production and Media Optimization from Bacillus subtilis. Rev. Chim.[internet]. 2020 Nov;71(11):1-9. Available from: https://doi.org/10.37358/RC.20.11.8368

APA 6th edition
Hussein, N.N.A., Ibrahim, A.I., Kamar, F.H. & Nechifor, A.C. (2020). Caseinase Production and Media Optimization from Bacillus subtilis. Revista de Chimie, 71(11), 1-9. https://doi.org/10.37358/RC.20.11.8368

Harvard
Hussein, N.N.A., Ibrahim, A.I., Kamar, F.H., Nechifor, A.C. (2020). 'Caseinase Production and Media Optimization from Bacillus subtilis', Revista de Chimie, 71(11), pp. 1-9. https://doi.org/10.37358/RC.20.11.8368

IEEE
N.N.A. Hussein, A.I. Ibrahim, F.H. Kamar, A.C. Nechifor, "Caseinase Production and Media Optimization from Bacillus subtilis". Revista de Chimie, vol. 71, no. 11, pp. 1-9, 2020. [online]. https://doi.org/10.37358/RC.20.11.8368

Text
Noor Nihad Abdul Hussein, Aseel I. Ibrahim, Firas Hashim Kamar, Arelia Cristina Nechifor,
Caseinase Production and Media Optimization from Bacillus subtilis,
Revista de Chimie,
Volume 71, Issue 11,
2020,
Pages 1-9,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.11.8368.
(https://revistadechimie.ro/Articles.asp?ID=8368)
Keywords: Caseinase; Bacillus subtilis; kinetic characterization; 16S rRNA sequencing

RIS
TY - JOUR
T1 - Caseinase Production and Media Optimization from Bacillus subtilis
A1 - Hussein, Noor Nihad Abdul
A2 - Ibrahim, Aseel I.
A3 - Kamar, Firas Hashim
A4 - Nechifor, Arelia Cristina
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 11
SP - 1
EP - 9
UR - https://doi.org/10.37358/RC.20.11.8368
KW - Caseinase
KW - Bacillus subtilis
KW - kinetic characterization
KW - 16S rRNA sequencing

ER -

BibTex
@article{RevCh2020P1,
author = {Hussein Noor Nihad Abdul and Ibrahim Aseel I. and Kamar Firas Hashim and Nechifor Arelia Cristina},
title = {Caseinase Production and Media Optimization from Bacillus subtilis},
journal = {Revista de Chimie},
volume = {71},
number = {11},
pages = {1-9},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.11.8368},
url = {https://revistadechimie.ro/Articles.asp?ID=8368}
}

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