Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats

Ramzan, Tayyeba; Aslam, Bilal; Muhammad, Faqir; Faisal, Muhammad Naeem; Hussain, Asif

Tayyeba Ramzan, Bilal Aslam, Faqir Muhammad, Muhammad Naeem Faisal, Asif Hussain

Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats

Abstract:

Ocimum sanctum L. is widely used as traditional remedy to manage hyperglycemia. This study was aimed to evaluate the effect of aqueous-methanolic extract of O. sanctum leaves (OSE) on the anti-diabetic activity of gliclazide in alloxan-induced diabetes in Wistar rats. Diabetes was induced by intraperitoneally injecting alloxan (120 mg/kg b.w.) in rats. Treatments including OSE (100 mg/kg b.w.), gliclazide (100 mg/kg b.w.), and in combination were given daily to diabetic rats till the 21st day of study. Body weight and fasting blood glucose levels were determined at regular intervals, while blood and organ samples were taken at the end of the study for biochemical and histopathological studies. Results showed that treatments exhibited anti-hyperglycemic activity through significantly (p [ 0.05) restoring body weight, fasting blood glucose level, and serum levels of glucose, insulin and HbA1c. The anti-lipidemic activity was noticed as total cholesterol (TC), triglyceride (TG), high-/low density-lipoproteins (HDL-C, LDL-C) levels were restored in treated diabetic rats. Ameliorative effects of treatments were observed as significant (p [ 0.05) reduction in serum levels of liver function biomarkers (alanine aminotransferase; ALT, aspartate aminotransferase; AST, alkaline phosphatase; ALP and bilirubin; BIL) and restoration of oxidative stress biomarkers (catalase; CAT, superoxide dismutase; SOD and malondialdehyde; MDA) in liver tissue. Histopathological findings supported these results as an increase in pancreatic islets size and protective effects on liver tissue was observed in diabetic rats treated with gliclazide and OSE alone and their combination. Conclusively, the combination of OSE and gliclazide produced a synergistic anti-diabetic effect as compared to that of alone treatment.

Keywords:
Ocimum sanctum; gliclazide; antioxidants; alloxan; oxidative stress; diabetes

Issue: 2020, Volume 71, Issue 11

Pages: 101-110

Publication date: 2020/12/4

https://doi.org/10.37358/RC.20.11.8379

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
RAMZAN, T., ASLAM, B., MUHAMMAD, F., FAISAL, M.N., HUSSAIN, A., Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats, Rev. Chim., 71(11), 2020, 101-110.

Vancouver
Ramzan T, Aslam B, Muhammad F, Faisal MN, Hussain A. Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats. Rev. Chim.[internet]. 2020 Nov;71(11):101-110. Available from: https://doi.org/10.37358/RC.20.11.8379

APA 6th edition
Ramzan, T., Aslam, B., Muhammad, F., Faisal, M.N. & Hussain, A. (2020). Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats. Revista de Chimie, 71(11), 101-110. https://doi.org/10.37358/RC.20.11.8379

Harvard
Ramzan, T., Aslam, B., Muhammad, F., Faisal, M.N., Hussain, A. (2020). 'Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats', Revista de Chimie, 71(11), pp. 101-110. https://doi.org/10.37358/RC.20.11.8379

IEEE
T. Ramzan, B. Aslam, F. Muhammad, M.N. Faisal, A. Hussain, "Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats". Revista de Chimie, vol. 71, no. 11, pp. 101-110, 2020. [online]. https://doi.org/10.37358/RC.20.11.8379

Text
Tayyeba Ramzan, Bilal Aslam, Faqir Muhammad, Muhammad Naeem Faisal, Asif Hussain,
Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats,
Revista de Chimie,
Volume 71, Issue 11,
2020,
Pages 101-110,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.11.8379.
(https://revistadechimie.ro/Articles.asp?ID=8379)
Keywords: Ocimum sanctum; gliclazide; antioxidants; alloxan; oxidative stress; diabetes

RIS
TY - JOUR
T1 - Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats
A1 - Ramzan, Tayyeba
A2 - Aslam, Bilal
A3 - Muhammad, Faqir
A4 - Faisal, Muhammad Naeem
A5 - Hussain, Asif
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 11
SP - 101
EP - 110
UR - https://doi.org/10.37358/RC.20.11.8379
KW - Ocimum sanctum
KW - gliclazide
KW - antioxidants
KW - alloxan
KW - oxidative stress
KW - diabetes
ER -

BibTex
@article{RevCh2020P101,
author = {Ramzan Tayyeba and Aslam Bilal and Muhammad Faqir and Faisal Muhammad Naeem and Hussain Asif},
title = {Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats},
journal = {Revista de Chimie},
volume = {71},
number = {11},
pages = {101-110},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.11.8379},
url = {https://revistadechimie.ro/Articles.asp?ID=8379}
}

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