Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design

Mulyani, Fitry; Permana, M. Diki; Ishmayana, Safri; Rahayu, Iman; Eddy, Diana Rakhmawaty

Fitry Mulyani, M. Diki Permana, Safri Ishmayana, Iman Rahayu, Diana Rakhmawaty Eddy

Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design

Abstract:

Zinc oxide nanoparticles have wide applications as catalysts, antimicrobial agents, drug delivery agents, etc. because of their intrinsic properties. Various methods can be applied to synthesise nanoparticles, one of which is the biosynthesis process. Biosynthesis is more eco-friendly than chemical and physical methods. In the present study, the optimisation of zinc oxide nanoparticle biosynthesis using the yeast Saccharomyces cerevisiae was performed by applying a response surface method called the Box-Behnken design (BBD). Three factors were optimised in the present study, namely the concentration of zinc acetate as the precursor (X1), concentration of the S. cerevisiae fermentation broth (X2), and the incubation time (X3). The mass of zinc oxide nanoparticles (Y) was recorded as the response of the experiment. The product was then characterised by fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD), scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS), and particle size analyser (PSA). The optimum conditions for the preparation of zinc oxide nanoparticles were found to be 0.3 M, 100% (v/v), and 24 h as the zinc acetate concentration, medium concentration, and incubation time, respectively. The FTIR analysis showed peaks at ~600 cm−1, which is characteristic for ZnO stretching. From the XRD result, the ZnO nanoparticles with hexagonal structure was confirm. The SEM/EDS analysis confirmed that the morphology was spherical and showed the major energy emission for zinc and oxygen. Moreover, the PSA analysis revealed that the smallest size was 218.6 nm (12%) when the synthesis was performed at the optimum conditions, while when the incubation time was prolonged for 120 h, the size decreased to 134.2 nm.

Keywords:
Biosynthesis; Box-Behnken; Saccharomyces cerevisiae; ZnO nanoparticles

Issue: 2021, Volume 72, Issue 1

Pages: 79-89

Publication date: 2021/2/3

https://doi.org/10.37358/RC.21.1.8405

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MULYANI, F., PERMANA, M.D., ISHMAYANA, S., RAHAYU, I., EDDY, D.R., Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design, Rev. Chim., 72(1), 2021, 79-89.

Vancouver
Mulyani F, Permana MD, Ishmayana S, Rahayu I, Eddy DR. Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design. Rev. Chim.[internet]. 2021 Jan;72(1):79-89. Available from: https://doi.org/10.37358/RC.21.1.8405

APA 6th edition
Mulyani, F., Permana, M.D., Ishmayana, S., Rahayu, I. & Eddy, D.R. (2021). Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design. Revista de Chimie, 72(1), 79-89. https://doi.org/10.37358/RC.21.1.8405

Harvard
Mulyani, F., Permana, M.D., Ishmayana, S., Rahayu, I., Eddy, D.R. (2021). 'Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design', Revista de Chimie, 72(1), pp. 79-89. https://doi.org/10.37358/RC.21.1.8405

IEEE
F. Mulyani, M.D. Permana, S. Ishmayana, I. Rahayu, D.R. Eddy, "Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design". Revista de Chimie, vol. 72, no. 1, pp. 79-89, 2021. [online]. https://doi.org/10.37358/RC.21.1.8405

Text
Fitry Mulyani, M. Diki Permana, Safri Ishmayana, Iman Rahayu, Diana Rakhmawaty Eddy,
Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design,
Revista de Chimie,
Volume 72, Issue 1,
2021,
Pages 79-89,
ISSN 2668-8212,
https://doi.org/10.37358/RC.21.1.8405.
(https://revistadechimie.ro/Articles.asp?ID=8405)
Keywords: Biosynthesis; Box-Behnken; Saccharomyces cerevisiae; ZnO nanoparticles

RIS
TY - JOUR
T1 - Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design
A1 - Mulyani, Fitry
A2 - Permana, M. Diki
A3 - Ishmayana, Safri
A4 - Rahayu, Iman
A5 - Eddy, Diana Rakhmawaty
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2021
VL - 72
IS - 1
SP - 79
EP - 89
UR - https://doi.org/10.37358/RC.21.1.8405
KW - Biosynthesis
KW - Box-Behnken
KW - Saccharomyces cerevisiae
KW - ZnO nanoparticles
ER -

BibTex
@article{RevCh2021P79,
author = {Mulyani Fitry and Permana M. Diki and Ishmayana Safri and Rahayu Iman and Eddy Diana Rakhmawaty},
title = {Optimisation of Zinc Oxide Nanoparticle Biosynthesis Using Saccharomyces Cerevisiae with Box-Behnken Design},
journal = {Revista de Chimie},
volume = {72},
number = {1},
pages = {79-89},
year = {2021},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.21.1.8405},
url = {https://revistadechimie.ro/Articles.asp?ID=8405}
}

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