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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2021, Volume 72, Issue 2, 38-49

https://doi.org/10.37358/RC.21.2.8418

Bilal Aslam, Asif Hussain

Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum

Abstract:

This study was aimed to phytochemically characterize and assess the antioxidant activities of 70% methanol extract and subsequent fractions of Cassia absus (L.) seeds, Gymnema sylvestre (L.) whole plant, Nigella sativa (L.) seeds, and Piper nigrum (L.) seeds. Powdered plant materials were extracted with 70% methanol and further fractionated with ethyl-acetate, n-butanol and the residual aqueous fraction. Phytochemical analysis was performed to detect different phytocompounds. Mineral compositions were quantified, and total phenolic and flavonoid contents were determined. The antioxidant potential of methanol extracts and fractions was assessed in vitro through estimating DPPH radical and superoxide anion scavenging activities and reducing power assay. Extraction yields ranging highest of N. sativa methanol extract (30.42±1.49%) and lowest of P. nigrum ethyl-acetate fraction (4.58±0.61%) were obtained. Results revealed that methanol extracts and fractions of selected plants contain phytocompounds such as alkaloids, flavonoids, phenols, glycosides, tannins, terpenoids, saponins, carbohydrates, fats and fixed oils. The mineral analysis showed considerable quantities of calcium (C. absus methanol extract: 372.454±3.633 mg/100g), magnesium (G. sylvestre methanol extract: 131.045±1.346 mg/100g), and zinc (N. sativa methanol extract: 36.019±0.284 mg/100 g) in all fractions while minor quantities of manganese, copper and cobalt were also found. Methanol extracts showed considerably higher total phenolic (N. sativa methanol extract: 179.71±2.14 mg GAE/g) and flavonoid (N. sativa methanol extract: 189.18±3.17 mg CE/g) contents compared to other fractions, and subsequently exhibited pronounced scavenging activities on DPPH* (N. sativa methanol extract: 23.8 µg/mL) and superoxide radicals (N. sativa methanol extract: 24.9 µg/mL) and had potent reductive abilities (N. sativa methanol extract: 1.123±0.038 O.D.). Conclusively, C. absus, G. sylvestre, N. sativa and P. nigrum possess significant nutritive properties and could be used as natural antioxidant sources to prevent oxidative stress-associated diseases.
Keywords:
antioxidants; Cassia absus; Gymnema sylvestre; Nigella sativa; Piper nigrum; polyphenols

Issue: 2021, Volume 72, Issue 2
Pages: 38-49
Publication date: 2021/5/7
https://doi.org/10.37358/RC.21.2.8418
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
ASLAM, B., HUSSAIN, A., Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum, Rev. Chim., 72(2), 2021, 38-49.

Vancouver
Aslam B, Hussain A. Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum. Rev. Chim.[internet]. 2021 Feb;72(2):38-49. Available from: https://doi.org/10.37358/RC.21.2.8418


APA 6th edition
Aslam, B., Hussain, A. (2021). Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum. Revista de Chimie, 72(2), 38-49. https://doi.org/10.37358/RC.21.2.8418


Harvard
Aslam, B., Hussain, A. (2021). 'Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum', Revista de Chimie, 72(2), pp. 38-49. https://doi.org/10.37358/RC.21.2.8418


IEEE
B. Aslam, A. Hussain, "Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum". Revista de Chimie, vol. 72, no. 2, pp. 38-49, 2021. [online]. https://doi.org/10.37358/RC.21.2.8418


Text
Bilal Aslam, Asif Hussain,
Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum,
Revista de Chimie,
Volume 72, Issue 2,
2021,
Pages 38-49,
ISSN 2668-8212,
https://doi.org/10.37358/RC.21.2.8418.
(https://revistadechimie.ro/Articles.asp?ID=8418)
Keywords: antioxidants; Cassia absus; Gymnema sylvestre; Nigella sativa; Piper nigrum; polyphenols


RIS
TY - JOUR
T1 - Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum
A1 - Aslam, Bilal
A2 - Hussain, Asif
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2021
VL - 72
IS - 2
SP - 38
EP - 49
UR - https://doi.org/10.37358/RC.21.2.8418
KW - antioxidants
KW - Cassia absus
KW - Gymnema sylvestre
KW - Nigella sativa
KW - Piper nigrum
KW - polyphenols
ER -


BibTex
@article{RevCh2021P38,
author = {Aslam Bilal and Hussain Asif},
title = {Phytochemical Characterization and Solvent Fraction Depending in vitro Antioxidant Activities of Cassia absus, Gymnema sylvestre, Nigella sativa and Piper nigrum},
journal = {Revista de Chimie},
volume = {72},
number = {2},
pages = {38-49},
year = {2021},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.21.2.8418},
url = {https://revistadechimie.ro/Articles.asp?ID=8418}
}
 
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