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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2021, Volume 72, Issue 2, 102-118

https://doi.org/10.37358/RC.21.2.8424

Amr M. Yehia, Mahmoud G. Eldeib, Ahmed M. Mohamadin, Mohammed M. El-Zahab

Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression

Abstract:

Owing to a lack of appropriate therapeutic regimens, prostate cancer (PC) is a global health concern with a high incidence and mortality rate in elderly men. Combination treatment seems to have the highest clinical benefit and avoids unwanted side effects. The current study focused on the chemotherapeutic efficacy of Zoledronic acid (ZA) in combination with 5-fluorouracil (5-FU) or Flutamide on prostate cancer cells, as well as its effect on apoptosis. The MTT assay was used to determine the cytotoxic effect of Zoledronic acid (ZA), 5-FU, and flutamide on PC-3 and DU-145 cells, as well as the combined therapy of ZA with 5-FU or flutamide. Additionally, immunofluorescence staining analysis was used to assess changes in Bcl-2 and p53 expression. Furthermore, the western blotting method was extensively used to evaluate Bax, caspase 3, and cyclin D1. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine the relative expression of miRNA-382 (miR-382) and miRNA-18a (miR-18a). Instead of (13.47, 8.23, and 9.42 iM) for PC-3 or (38.77, 17.6, and 8.47 iM) for DU145 cells, the combination therapy improved cytotoxicity with doses approximately half of IC50 (6.74, 4.12, and 7.07 iM) in PC-3 and (19.38, 8.8, and 6.33 iM) in DU145 cells for ZA, 5-FU, and flutamide, respectively. When compared to a single therapy, the combination therapy significantly up-regulated the pro-apoptotic Bax, cleaved caspase 3 and p53 levels while down-regulated the cyclin D1 and Bcl-2 expression. In addition, the combination therapy was linked to changes in miR-382 and miR-18a expression. Our findings suggest that combining ZA with 5-FU or flutamide improves chemotherapeutic efficacy against prostate cancer cells, at least in part by encouraging apoptosis and modulating miRNA expression, especially miR-382 and miR-18a.
Keywords:
5-Fluorouracil (5-FU); Flutamide; miRNAs; PC-3; Prostate cancer; Zoledronic acid

Issue: 2021, Volume 72, Issue 2
Pages: 102-118
Publication date: 2021/5/7
https://doi.org/10.37358/RC.21.2.8424
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
YEHIA, A.M., ELDEIB, M.G., MOHAMADIN, A.M., EL-ZAHAB, M.M., Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression, Rev. Chim., 72(2), 2021, 102-118.

Vancouver
Yehia AM, Eldeib MG, Mohamadin AM, El-Zahab MM. Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression. Rev. Chim.[internet]. 2021 Feb;72(2):102-118. Available from: https://doi.org/10.37358/RC.21.2.8424


APA 6th edition
Yehia, A.M., Eldeib, M.G., Mohamadin, A.M. & El-Zahab, M.M. (2021). Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression. Revista de Chimie, 72(2), 102-118. https://doi.org/10.37358/RC.21.2.8424


Harvard
Yehia, A.M., Eldeib, M.G., Mohamadin, A.M., El-Zahab, M.M. (2021). 'Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression', Revista de Chimie, 72(2), pp. 102-118. https://doi.org/10.37358/RC.21.2.8424


IEEE
A.M. Yehia, M.G. Eldeib, A.M. Mohamadin, M.M. El-Zahab, "Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression". Revista de Chimie, vol. 72, no. 2, pp. 102-118, 2021. [online]. https://doi.org/10.37358/RC.21.2.8424


Text
Amr M. Yehia, Mahmoud G. Eldeib, Ahmed M. Mohamadin, Mohammed M. El-Zahab,
Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression,
Revista de Chimie,
Volume 72, Issue 2,
2021,
Pages 102-118,
ISSN 2668-8212,
https://doi.org/10.37358/RC.21.2.8424.
(https://revistadechimie.ro/Articles.asp?ID=8424)
Keywords: 5-Fluorouracil (5-FU); Flutamide; miRNAs; PC-3; Prostate cancer; Zoledronic acid


RIS
TY - JOUR
T1 - Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression
A1 - Yehia, Amr M.
A2 - Eldeib, Mahmoud G.
A3 - Mohamadin, Ahmed M.
A4 - El-Zahab, Mohammed M.
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2021
VL - 72
IS - 2
SP - 102
EP - 118
UR - https://doi.org/10.37358/RC.21.2.8424
KW - 5-Fluorouracil (5-FU)
KW - Flutamide
KW - miRNAs
KW - PC-3
KW - Prostate cancer
KW - Zoledronic acid
ER -


BibTex
@article{RevCh2021P102,
author = {Yehia Amr M. and Eldeib Mahmoud G. and Mohamadin Ahmed M. and El-Zahab Mohammed M.},
title = {Zoledronic Acid Enhances the Chemotherapeutic Efficiency of 5-fluorouracil or Flutamide in Prostate Cancer Cells with Modulation of miR-382 and miR-18a Expression},
journal = {Revista de Chimie},
volume = {72},
number = {2},
pages = {102-118},
year = {2021},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.21.2.8424},
url = {https://revistadechimie.ro/Articles.asp?ID=8424}
}
 
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